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United States (US)

K562 whole cell lysate

Cat. No. GTX27911

Species

Human
Package
100 μg ($239)
PRODUCT

Summary

K562 lysate was prepared by homogenization in modified RIPA buffer. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The lysate was boiled for 5 min in 1 x SDS sample buffer containing 5% β-mercaptoethanol.

PROPERTIES

Form

Liquid

Buffer

1x SDS sample buffer (50mM Tris-HCl, 12.5% Glycerol, 1% Sodium Dodecylsulfate, 0.01% Bromophenol blue), 5% β-mercaptoethanol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2 mg/ml (Please refer to the vial label for the specific concentration.)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

REFERENCE

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SDS
Glycerol.pdf
Package List Price ($)
$ 239