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KLF5 antibody

Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289
Anti-KLF5 antibody used in Western Blot (WB). GTX103289

Cat. No. GTX103289

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

51 kDa. ( Note )

Positive Control

KLF5-transfected 293T , HeLa (500 μM CoCl2 treatment for 24 hr and 10 μM MG132 treatment for 2 hr)

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human KLF5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950625

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Kruppel like factor 5 , BTEB2 , CKLF , IKLF

Cellular Localization

Nucleus

Background

This gene encodes a member of the Kruppel-like factor subfamily of zinc finger proteins. Since the protein localizes to the nucleus and binds the epidermal growth factor response element, it is thought to be a transcription factor. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Wild-type (WT) and KLF5 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Untreated (–) and treated (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus on HeLa xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded HeLa xenograft.
KLF5 antibody (GTX103289) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103289

GTX103289 ICC/IF Image

KLF5 antibody detects KLF5 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody used in IHC (Paraffin sections) (IHC-P). GTX103289

GTX103289 IHC-P Image

KLF5 antibody detects KLF5 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
KLF5 stained by KLF5 antibody (GTX103289) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-KLF5 antibody used in Western Blot (WB). GTX103289

GTX103289 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with KLF5 antibody (GTX103289) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

KLF5 antibody Cat. No. GTX103289

Rating ( Average 4.8 based on 10 users reviews)
Application
Western Blot(WB) ( Average 5 based on 8 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 3 based on 1 users reviews)
Immunoprecipitation(IP) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014. PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biosci Rep in 2014. PMID: 25205373
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biosci Rep in 2014. PMID: 25205373
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014. PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB,IP
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014. PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014. PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014. PMID: 25197166
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Biosci Rep in 2014. PMID: 25205373
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Mediators Inflamm in 2014. PMID: 25197166
Date : Anonymous submitted on 22-Nov-2017
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : mouse heart tissue
Blocking : 3%BSA in PBST, 25°C, 1Hr
Fixation : 4%PFA in PBS
Primary Antibody : 1:1500, 4°C, 15Hr
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169