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1 .GTX26526 Ki67 antibody [PP-67]

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Ki67 antibody [PP-67]


  • Host / ClonalityMouse Monoclonal
  • Clone NamePP-67
  • ApplicationsICC/IF, IHC-P
  • Species reactivityHuman, Mouse
  • Publication  ( 2 )
Cat No: GTX26526
Package Price Qty
100 μlUSD 289.00
Currently In Production.
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  • Anti-Ki67 antibody [PP-67] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26526
  • Anti-Ki67 antibody [PP-67] used in IHC (Formalin-fixed paraffin-embedded sections). GTX26526
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  • Application Information: Ki67 antibody [PP-67]

Catalog Number GTX26526
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Publication ( 2 )
Product Name Ki67 antibody [PP-67]
See all Ki67 products
ApplicationsICC/IF, IHC-P
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)Assay-dependent dilution Y
IHC (Formalin-fixed paraffin-embedded sections)1:800* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Positive Controlshuman tonsil
Form SuppliedLiquid
PurificationUnpurified

  • Specifications: Ki67 antibody [PP-67]

Full Namemarker of proliferation Ki-67
BackgroundKi-67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. The monoclonal antibody is useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labeling index. The correlation between low Ki67 index and histologically low-grade tumors is strong.
SynonymsKIA
SpecificityReacts with the Ki-67 nuclear antigen (345 and 395 kDa, and several lower m.w. proteins) in immunoblotting. The product shows an immunohistochemical staining pattern similar to that obtained with other anti-Ki-67/MIB-1 reagents. It labels proliferating cells in frozen human tissue and in formalin-fixed, paraffin-embedded tissue. A microwave heating retrieval step is required for successful labeling of routinely formalin-fixed, paraffin-embedded human tissue.
HostMouse
ClonalityMonoclonal
Clone NamePP-67
IsotypeIgM
ImmunogenHeLa cell nuclear preparation.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
ConjugationUnconjugated

  • Storage Conditions: Ki67 antibody [PP-67]

Storage BufferAscites, 15 mM sodium azide
Storage InstructionKeep as concentrated solution. For short-term storage, store at 4° C (up to 10 days). For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Tumor biomarkers
  • Cancer > Type of cancer > Skin > Squamous cell
  • Cell Biology > Cell cycle
  • Disease Related > Alzheimer's disease
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Ki67 antibody [PP-67] validated data
  • Anti-Ki67 antibody [PP-67] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26526
    • GTX26526 ICC/IF Image
      ICC/IF analysis of SK-MEL-28 cells using GTX26526 Ki67 antibody [PP-67] at 1:200. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100.
      Top
  • Anti-Ki67 antibody [PP-67] used in IHC (Formalin-fixed paraffin-embedded sections). GTX26526
    • GTX26526 IHC-P Image
      IHC-P analysis of human tonsil sections using GTX26526 Ki67 antibody [PP-67] at 1:800.
      Top
  • Anti-Ki67 antibody [PP-67] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX26526
    • GTX26526 ICC/IF Image
      ICC/IF analysis of SK-MEL-28 cells using GTX26526 Ki67 antibody [PP-67] at 1:200. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100.
      Top
  • Anti-Ki67 antibody [PP-67] used in IHC (Formalin-fixed paraffin-embedded sections). GTX26526
    • GTX26526 IHC-P Image
      IHC-P analysis of human tonsil sections using GTX26526 Ki67 antibody [PP-67] at 1:800.
      Top
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  • Reference for Ki67 antibody [PP-67]
Clone References Application Reactivity
Roth Flach RJ et al. Protein Kinase Mitogen-activated Protein Kinase Kinase Kinase Kinase 4 (MAP4K4) Promotes Obesity-induced Hyperinsulinemia. J Biol Chem 291 (31):16221-30 (2016). IHC-P Mouse
Xu M et al. Chromatin assembly factor 1, subunit A (P150) facilitates cell proliferation in human hepatocellular carcinoma. Onco Targets Ther 9:4023-35 (2016). ICC/IF Human
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