| Full Name | marker of proliferation Ki-67 |
| Background | Ki-67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. The monoclonal antibody is useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67-positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine-labeling index. The correlation between low Ki67 index and histologically low-grade tumors is strong. |
| Synonyms | KIA |
| Specificity | Reacts with the Ki-67 nuclear antigen (345 and 395 kDa, and several lower m.w. proteins) in immunoblotting. The product shows an immunohistochemical staining pattern similar to that obtained with other anti-Ki-67/MIB-1 reagents. It labels proliferating cells in frozen human tissue and in formalin-fixed, paraffin-embedded tissue. A microwave heating retrieval step is required for successful labeling of routinely formalin-fixed, paraffin-embedded human tissue. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone Name | PP-67 |
| Isotype | IgM |
| Immunogen | HeLa cell nuclear preparation. |
| Antigen Species | Human |
| Species Reactivity | Human, Mouse |
| Conjugation | Unconjugated |