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Ku70 antibody [529]

Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607
Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607
Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607
Anti-Ku70 antibody [529] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77607

Cat. No. GTX77607

Host

Mouse

Clonality

Monoclonal

Clone Name

529

Isotype

IgG1

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

70 kDa. ( Note )

Positive Control

Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant antigen containing the amino terminal portion of human Ku70 protein

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_424561

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

X-ray repair cross complementing 6 , CTC75 , CTCBF , G22P1 , KU70 , ML8 , TLAA

Cellular Localization

Nucleus

Background

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607

GTX77607 WB Image

Detection of human Ku70 protein in HeLa whole cell lysate using GeneTex Ku70 529 monoclonal antibody GTX77607.

Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607

GTX77607 WB Image

Sample (30 ug of whole cell lysate)
A: Jurkat
B: Raji
C: 293T
D: A431
E: HeLa
F: HepG2
G: H1299
H: HCT116
I: MCF-7
J: NT2D1
K: PC-3
L: U87-MG
7.5% SDS PAGE
GTX77607 diluted at 1:1000

Anti-Ku70 antibody [529] used in Western Blot (WB). GTX77607

GTX77607 WB Image

Sample (whole cell lysate)
A: 293T 20ug
B: 293T 10ug
C: 293T 5ug
7.5% SDS PAGE
GTX77607 diluted at 1:5000

Anti-Ku70 antibody [529] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX77607

GTX77607 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Ku70(GTX77607) antibody (green) at 1:2000 dilution. Alpha-tubulin filaments were labeled with GTX11304 (red) at 1:2000.

REFERENCE

REVIEW

Ku70 antibody [529] Cat. No. GTX77607

Rating ( Average 5 based on 3 users reviews)
Application
Western Blot(WB) ( Average 5 based on 3 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019.PMID: 31601897
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31164689
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Sci Rep in 2019. PMID: 31164689
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations