GeneTex
  • Country / Location Selection

United States (US)

LAMP2 antibody

Anti-LAMP2 antibody used in Western Blot (WB). GTX30881
Anti-LAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30881
Anti-LAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX30881

Cat. No. GTX30881

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, ELISA

Reactivity

Human, Mouse
Package
50 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
ICC/IF 10 μg/mL
IHC-P Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Hep G2 Cell Slide(GTX25530) , HepG2 Cell Lysate(GTX27900)

Product Note

LAMP-2 antibody cross reacts with LAMP2B and LAMP2C, but not LAMP2A.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

LAMP-2 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human LAMP-2.The immunogen is located within the last 50 amino acids of LAMP-2.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_1952757

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysosomal associated membrane protein 2 , CD107b , LAMP-2 , LAMPB , LGP-96 , LGP110

Cellular Localization

Cell membrane,Endosome membrane,Lysosome membrane

Background

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). LAMP-2, a highly glycosylated protein associated with the lysosome (4), has recently been shown to be important in autophagy as mice deficient in this protein failed to convert autophagic vacuoles into vacuoles (5) leading to impaired degradation of long-lived proteins. This correlates with the finding that human LAMP-2 deficiency causing DanonOs disease is associated with the accumulation of autophagic material in striated myocytes (6). LAMP-2 exists in multiple isoforms (7).

Database

Research Area

DATA IMAGES

Anti-LAMP2 antibody used in Western Blot (WB). GTX30881

GTX30881 WB Image

WB analysis of HepG2 cell lysate using GTX30881 LAMP2 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-LAMP2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30881

GTX30881 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX30881 LAMP2 antibody.
Working concentration : 10 μg/ml

Anti-LAMP2 antibody used in IHC (Paraffin sections) (IHC-P). GTX30881

GTX30881 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX30881 LAMP2 antibody.
Dilution : 5 μg/ml

REFERENCE

REVIEW

There are currently no reviews for LAMP2 antibody (GTX30881). Be the first to share your experience with this product.
SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399