|Applications||FACS, ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||15 kDa (note)
|Positive Controls||NT2D1 , PC-3 , U87-MG , SK-N-SH , mouse brain , Rat brain , HepG2 (untreated) , HepG2 (3 μM Thapsigargin treatment for 12 hr) , HepG2 (3 μM Thapsigargin treatment for 16hr) , HepG2 (3 μM Thapsigargin treatment for 24hr) , Huh7 (un-infected) , Huh7 (HCV-infected) , HeLa , HeLa (50μM Chloroquine treatment for 24 hr)|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||microtubule-associated protein 1 light chain 3 beta|
|Product Description||Rabbit Polyclonal antibody to LC3B (microtubule-associated protein 1 light chain 3 beta)|
|Background|| The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq]|
|Synonyms||ATG8F antibody, LC3B antibody, MAP1A/1BLC3 antibody, MAP1LC3B antibody, autophagy-related ubiquitin-like modifier LC3 B antibody, MAP1A/MAP1B LC3 B antibody, MAP1A/MAP1B light chain 3 B antibody, MAP1 light chain 3-like protein 2 antibody, microtubule-associated proteins 1A/1B light chain 3B antibody, microtubule-associated protein 1 light chain 3 beta antibody|
|Cellular Localization||Cytoplasm , Endomembrane system; Lipid-anchor , Cytoplasmic vesicle , autophagosome membrane; Lipid-anchor |
|Specificity||Cross-reactivity may occur with other LC3 isoforms|
|Immunogen||Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human LC3B. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Pig, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Japanese Medaka, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Zebrafish (100%), Japanese Medaka (100%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%)|