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LIMCH1 antibody [C2C3], C-term

KO/KD Orthogonal
Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649
Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649
Anti-LIMCH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108649
Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649
Anti-LIMCH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108649

Cat. No. GTX108649

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all LIMCH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

mouse brain , HeLa

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.53 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human LIMCH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950813

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LIM and calponin homology domains 1 , LIMCH1A , LMO7B

Database

Research Area

DATA IMAGES

Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649

GTX108649 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649

GTX108649 WB Image

(30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIMCH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX108649

GTX108649 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LIMCH1(GTX108649) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-LIMCH1 antibody [C2C3], C-term used in Western Blot (WB). GTX108649

GTX108649 WB Image

KO/KD

Wild-type (WT) and LIMCH1 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-LIMCH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX108649

GTX108649 ICC/IF Image

LIMCH1 antibody [C2C3], C-term detects LIMCH1 protein at stress fiber by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LIMCH1 stained by LIMCH1 antibody [C2C3], C-term (GTX108649) diluted at 1:500.

REFERENCE

Application Reference

Krause K et al. Neuropathol Appl Neurobiol 2023; 49 (1) : e12853 Target formation in muscle fibres indicates reinnervation - A proteomic study in muscle samples from peripheral neuropathies
Application : WB
Reactivity : Human, Mouse
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169