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  • LIN7B antibody [1G1]

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1 .GTX84217 LIN7B antibody [1G1]

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LIN7B antibody [1G1]


  • Host / ClonalityMouse Monoclonal
  • Clone Name1G1
  • ApplicationsFACS, ICC/IF, WB
  • Species reactivityHuman
Cat No: GTX84217
Package Price Qty
100 μlUSD 339.00
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  • Anti-LIN7B antibody [1G1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84217
  • Anti-LIN7B antibody [1G1] used in Western Blot (WB). GTX84217
  • Anti-LIN7B antibody [1G1] used in Flow cytometry/FACS. GTX84217
  • Anti-LIN7B antibody [1G1] used in Flow cytometry/FACS. GTX84217
  • Anti-LIN7B antibody [1G1] used in Flow cytometry/FACS. GTX84217
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  • Datasheet
  • Application Information: LIN7B antibody [1G1]

Catalog Number GTX84217
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Product Name LIN7B antibody [1G1]
See all LIN7B products
ApplicationsFACS, ICC/IF, WB
Application NoteRecommended Starting Dilutions:

WB 1:2000,
Optimal working dilutions should be determined experimentally by the end user.

Form SuppliedLiquid
PurificationProtein A/G purified

  • Specifications: LIN7B antibody [1G1]

Full Namelin-7 homolog B (C. elegans)
BackgroundPlays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ACCN3 acid-evoked currents by stabilizing the channel at the cell surface.
SynonymsLIN7B, LIN-7B, MALS-2, VELI2, MALS2, LIN 7B, MALS 2
Cellular LocalizationEnriched in synaptosomes and at epithelial cell-cell junctions (By similarity). Mainly basolateral in renal epithelial cells.
SpecificityGTX84217 is specific for human LIN7B (NP_071448).
HostMouse
ClonalityMonoclonal
Clone Name1G1
IsotypeIgG1
ImmunogenFull length human recombinant protein of human LIN7B (NP_071448) produced in HEK293T cell.
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: LIN7B antibody [1G1]

Storage BufferPBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Storage InstructionFor short-term storage, store at 4°C or aliquot into working amounts and store at -20°C. For long-term storage, store at -70°C (aliquotted). Avoid repeated freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cell Biology > Exocytosis
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LIN7B antibody [1G1] validated data
  • Anti-LIN7B antibody [1G1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84217
    • GTX84217 ICC/IF Image
      Anti-LIN7B mouse monoclonal antibody (GTX84217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIN7B
      Top
  • Anti-LIN7B antibody [1G1] used in Western Blot (WB). GTX84217
    • GTX84217 WB Image
      HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIN7B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIN7B.
      Top
  • Anti-LIN7B antibody [1G1] used in Flow cytometry/FACS. GTX84217
    • GTX84217 FACS Image
      Flow cytometric Analysis of Hela cells, using anti-LIN7B antibody(GTX84217),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
      Top
  • Anti-LIN7B antibody [1G1] used in Flow cytometry/FACS. GTX84217
    • GTX84217 FACS Image
      Flow cytometric Analysis of Jurkat cells, using anti-LIN7B antibody(GTX84217),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
      Top
  • Anti-LIN7B antibody [1G1] used in Flow cytometry/FACS. GTX84217
    • GTX84217 FACS Image
      HEK293T cells transfected with either RC205598 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIN7B antibody(GTX84217), and then analyzed by flow cytometry.
      Top
  • Anti-LIN7B antibody [1G1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84217
    • GTX84217 ICC/IF Image
      Anti-LIN7B mouse monoclonal antibody (GTX84217) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY LIN7B
      Top
  • Anti-LIN7B antibody [1G1] used in Western Blot (WB). GTX84217
    • GTX84217 WB Image
      HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIN7B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIN7B.
      Top
  • Anti-LIN7B antibody [1G1] used in Flow cytometry/FACS. GTX84217
    • GTX84217 FACS Image
      Flow cytometric Analysis of Hela cells, using anti-LIN7B antibody(GTX84217),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
      Top
  • Anti-LIN7B antibody [1G1] used in Flow cytometry/FACS. GTX84217
    • GTX84217 FACS Image
      Flow cytometric Analysis of Jurkat cells, using anti-LIN7B antibody(GTX84217),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
      Top
  • Anti-LIN7B antibody [1G1] used in Flow cytometry/FACS. GTX84217
    • GTX84217 FACS Image
      HEK293T cells transfected with either RC205598 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-LIN7B antibody(GTX84217), and then analyzed by flow cytometry.
      Top
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