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LKB1 (phospho Ser428) antibody

Orthogonal
Anti-LKB1 (phospho Ser428) antibody used in Western Blot (WB). GTX30765
Anti-LKB1 (phospho Ser428) antibody used in IHC (Paraffin sections) (IHC-P). GTX30765

Cat. No. GTX30765

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
50 μl ($399)
See all LKB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human LKB1 around the phosphorylation site of serine 428 (R-L-Sp-A-C).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_1952763

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

LKB1,PJS,STK11,hLKB1,serine/threonine kinase 11

Cellular Localization

Nucleus,Cytoplasm,Membrane,Mitochondrion

Background

This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-LKB1 (phospho Ser428) antibody used in Western Blot (WB). GTX30765

GTX30765 WB Image

Orthogonal

WB analysis of CV-1 cells treated with forsklin lysate using GTX30765 LKB1 (phospho Ser428) antibody.
Left : Primary antibody
Right : Primary antibody preincubated with immunogen peptide

Anti-LKB1 (phospho Ser428) antibody used in IHC (Paraffin sections) (IHC-P). GTX30765

GTX30765 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX30765 LKB1 (phospho Ser428) antibody.

REFERENCE

Application Reference

Jin C et al. Food Chem Toxicol 2021; 154 : 112350 LKB1/AMPKα signaling pathway and mitochondrial fission/fusion dynamics regulate apoptosis induced by 3-chlorpropane-1,2-diol in HEK293?cells.
Jiang WP et al. Antioxidants (Basel) 2021; 10 (6) Sanghuangporus sanghuang Mycelium Prevents Paracetamol-Induced Hepatotoxicity through Regulating the MAPK/NF-κB, Keap1/Nrf2/HO-1, TLR4/PI3K/Akt, and CaMKKβ/LKB1/AMPK Pathways and Suppressing Oxidative Stress and Inflammation.
Application : WB
Reactivity : Mouse
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
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Package List Price ($)
$ 399