Lamin B1 antibody

Knockout/Knockdown validated

Host / ClonalityRabbit Polyclonal
ApplicationsIHC-P, IP, WB
Species reactivityHuman, Hamster
★★★★★ ( 2 ) ( 11 )

Cat No:

GTX103292

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Star Review (2)
Reference (11)
Bio-info
Protocol & MSDS

Application Information: Lamin B1 antibody

Catalog Number

GTX103292

Product Name

Lamin B1 antibody

See all Lamin B1 products

Applications

IHC-P, IP, WB

Application Note

	Suggested dilution	Reference
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Immunoprecipitation	1:100-1:500*
Western Blot (WB)	1:500-1:20000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

66 kDa (note) IsoForm

(2)

Positive Controls

293T , A431 , HeLa , HepG2 , *Huh7

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Lamin B1 antibody

Full Name	lamin B1
Product Description	Rabbit Polyclonal antibody to Lamin B1 (lamin B1)
Background	The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq]
Synonyms	ADLD antibody, LMN antibody, LMN2 antibody, LMNB antibody, MGC111419 antibody, LMNB1 antibody, lamin-B1 antibody, lamin B1 antibody
Cellular Localization	Nucleus inner membrane; Lipid-anchor; Nucleoplasmic side
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Recombinant protein encompassing a sequence within the center region of human Lamin B1. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Hamster
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Mouse, Rat, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, Bovine
Predict Reactivity Note	Mouse (98%), Rat (98%), Xenopus laevis (85%), Chicken (91%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (99%)
Conjugation	Unconjugated

Storage Conditions: Lamin B1 antibody

Storage Buffer	0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Lamin B1 antibody validated data

GTX103292 WB Image

Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CRISPR knockout cell lines generated by

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GTX103292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Lamin B1 antibody(GTX103292) antibody at 1:250 dilution.

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GTX103292 IP Image

Immunoprecipitation of Lamin B1 protein from 293T whole cell extracts using 5 μg of Lamin B1 antibody (GTX103292).
Western blot analysis was performed using Lamin B1 antibody (GTX103292).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

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GTX103292 WB Image

Lamin B1 antibody detects Lamin B1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX103292 WB Image

Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

CRISPR knockout cell lines generated by

Top

GTX103292 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Lamin B1 antibody(GTX103292) antibody at 1:250 dilution.

Top

GTX103292 IP Image

Immunoprecipitation of Lamin B1 protein from 293T whole cell extracts using 5 μg of Lamin B1 antibody (GTX103292).
Western blot analysis was performed using Lamin B1 antibody (GTX103292).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Top

GTX103292 WB Image

Lamin B1 antibody detects Lamin B1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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StarReview
Lamin B1 antibody
Rating : ★★★★★ ( Average 5.0 based on 2 users reviews)
Western Blot (WB)(2) :★★★★☆

Anonymous StarReview submitted on 04 Jun 2015
Score : ★★★★☆
Application Tested : WB
Sample Species : Human
Sample : Huh7 cells
Amount used(ug/cells) : 3*10^4 cells
Blocking conditions
Blocking Buffer : 4% Block Ace
Incubation Time & Temperature : 26 (℃)/1hr
Primary antibody
Dilution : 1:500( conc. of dilution )
Incubation Time & Temperature : 26 (℃)/1hr
Data Description
Lane 1: Huh7 nuclear extract.Lane 2: Huh7 cytoplasmic extract.

Anonymous StarReview submitted on 05 Jul 2017
Score : ★★★★★
Application Tested : WB
Sample Species : Hamster
Sample : CHO-K1 cells
Blocking conditions
Blocking Buffer : 5% milk
Incubation Time & Temperature : 25 (℃)/1 hr
Primary antibody
Dilution : 1: 1000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight

Reference for Lamin B1 antibody

Application References	Application	Reactivity
Tsai TH et al. Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells. Molecules 22 (5) (2017).	WB	Human
Nickel S et al. Expression and Activity of Breast Cancer Resistance Protein (BCRP/ABCG2) in Human Distal Lung Epithelial Cells In Vitro. Pharm Res (Epub ahead of print) (2017).	WB	Human
Chao W et al. 3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways. Molecules 22 (4) (2017).	WB	Human
Zanini IM et al. Human Cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion. J Cell Sci (Epub ahead of print) (2017).	WB	Human
Choi JH et al. Characterization of MUDENG, a novel anti-apoptotic protein. Oncogenesis 5:e221 (2016).	WB	Human
Santoro A et al. SERPINE2 Inhibits IL-1α-Induced MMP-13 Expression in Human Chondrocytes: Involvement of ERK/NF-κB/AP-1 Pathways. PLoS One 10 (8):e0135979 (2015).	WB	Human
Mandal SM et al. Amphotericin B and anidulafungin directly interact with DNA and induce oxidative damage in the mammalian genome. Mol Biosyst 11 (9):2551-9 (2015).	WB	Human
Tseng CK et al. Aqueous extract of Gracilaria tenuistipitata suppresses LPS-induced NF-κB and MAPK activation in RAW 264.7 and rat peritoneal macrophages and exerts hepatoprotective effects on carbon tetrachloride-treated rat. PLoS One 9 (1):e86557 (2014).	WB	Rat
Sobolesky PM et al. Regulation of the tumor suppressor FOXO3 by the thromboxane-A2 receptors in urothelial cancer. PLoS One 9 (9):e107530 (2014).
Chang YL et al. Regulation of estrogen receptor α function in oral squamous cell carcinoma cells by FAK signaling. Endocr Relat Cancer (2014).
Li HC et al. Nuclear export and import of human hepatitis B virus capsid protein and particles. PLoS Pathog 6 (10):e1001162 (2010).		Human

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	LMNB1	4001	P20700, B2R6J6	150340, 169500	NM_001198557	NP_001185486	43.03
Homo sapiens	LMNB1	4001	P20700, B2R6J6	150340, 169500	NM_005573	NP_005564	66.42

Cross-react Bioinfo
Species	Gene name	Gene ID	SwissProt	Isoform (mRNA)	Protein Accession#	MW
Rattus norvegicus	Lmnb1	116685	P70615	NM_053905	NP_446357	66.62
Mus musculus	Lmnb1	16906	P14733	NM_010721	NP_034851	66.8

*MSDS

MSDS-Glycerol.pdf

MSDS-Thimerosal.pdf

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