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1 .GTX103292 Lamin B1 antibody

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Lamin B1 antibody

Knockout/Knockdown

  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, IP, WB
  • Species reactivityHuman, Rat, Hamster
  • ★★★★★   (  2  ) Publication  ( 18 )
Cat No: GTX103292
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292
  • Anti-Lamin B1 antibody used in Immunoprecipitation. GTX103292
  • Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292
  • Anti-Lamin B1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX103292
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  • Datasheet
  • Star Review (2)
    Reference (18)
  • Bio-info
  • Protocol & MSDS
  • Application Information: Lamin B1 antibody

Catalog Number GTX103292
★★★★★
( 2 )
Publication ( 18 )
Product Name Lamin B1 antibody
See all Lamin B1 products
ApplicationsIHC-P, IP, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:100-1:500*  
Western Blot (WB)1:500-1:20000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size66 kDa (note) IsoForm (2)
Positive Controls293T , A431 , HeLa , HepG2 , *Huh7
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Lamin B1 antibody

Full Namelamin B1
BackgroundThe nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. [provided by RefSeq]
SynonymsADLD antibody, LMN antibody, LMN2 antibody, LMNB antibody, MGC111419 antibody, LMNB1 antibody, lamin-B1 antibody, lamin B1 antibody
Cellular LocalizationNucleus inner membrane; Lipid-anchor; Nucleoplasmic side
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human Lamin B1. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Rat, Hamster
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Xenopus laevis, Chicken, Rhesus Monkey, Chimpanzee, Bovine
Predict Reactivity NoteMouse (98%), Xenopus laevis (85%), Chicken (91%), Rhesus Monkey (100%), Chimpanzee (99%), Bovine (99%)
ConjugationUnconjugated

  • Storage Conditions: Lamin B1 antibody

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Lamin B1 antibody validated data
  • Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292
    • GTX103292 WB Image
      Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-Lamin B1 antibody used in Immunoprecipitation. GTX103292
    • GTX103292 IP Image
      Immunoprecipitation of Lamin B1 protein from 293T whole cell extracts using 5 μg of Lamin B1 antibody (GTX103292).
      Western blot analysis was performed using Lamin B1 antibody (GTX103292).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292
    • GTX103292 WB Image
      Lamin B1 antibody detects Lamin B1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Lamin B1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX103292
    • GTX103292 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Lamin B1 antibody(GTX103292) antibody at 1:250 dilution.
      Top
  • Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292
    • GTX103292 WB Image
      Wild-type (WT) and Lamin B1 knockout (KO) HeLa cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      CRISPR knockout cell lines generated by www.edigene.com
      Top
  • Anti-Lamin B1 antibody used in Immunoprecipitation. GTX103292
    • GTX103292 IP Image
      Immunoprecipitation of Lamin B1 protein from 293T whole cell extracts using 5 μg of Lamin B1 antibody (GTX103292).
      Western blot analysis was performed using Lamin B1 antibody (GTX103292).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-Lamin B1 antibody used in Western Blot (WB). GTX103292
    • GTX103292 WB Image
      Lamin B1 antibody detects Lamin B1 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Lamin B1 antibody (GTX103292) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Lamin B1 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX103292
    • GTX103292 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Lamin B1 antibody(GTX103292) antibody at 1:250 dilution.
      Top
  • StarReview
  • Lamin B1 antibody

  • Rating : ★★★★★ ( Average 5.0 based on 2 users reviews)
  • Western Blot (WB)(2) :★★★★☆
  • Anonymous StarReview submitted on 04 Jun 2015
  • Score : ★★★★☆
  • Application Tested : WB
  • Sample Species : Human
  • Sample : Huh7 cells
  • Amount used(ug/cells) : 3*10^4 cells
  • Blocking conditions
  • Blocking Buffer : 4% Block Ace
  • Incubation Time & Temperature : 26 (℃)/1hr
  • Primary antibody
  • Dilution : 1:500( conc. of dilution )
  • Incubation Time & Temperature : 26 (℃)/1hr
  • Data Description
  • Lane 1: Huh7 nuclear extract.Lane 2: Huh7 cytoplasmic extract.
  • Anonymous StarReview submitted on 05 Jul 2017
  • Score : ★★★★★
  • Application Tested : WB
  • Sample Species : Hamster
  • Sample : CHO-K1 cells
  • Blocking conditions
  • Blocking Buffer : 5% milk
  • Incubation Time & Temperature : 25 (℃)/1 hr
  • Primary antibody
  • Dilution : 1: 1000( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/overnight
Back to Top
  • Reference for Lamin B1 antibody
Application References Application Reactivity
Chin YT et al. Thyroxine inhibits resveratrol-caused apoptosis by PD-L1 in ovarian cancer cells. Endocr Relat Cancer 25 (5):533-545 (2018). WB Human
Conde J et al. E74-Like Factor (ELF3) and Leptin, a Novel Loop Between Obesity and Inflammation Perpetuating a Pro-Catabolic State in Cartilage. Cell Physiol Biochem 45 (6):2401-2410 (2018). WB Human
Chen Q et al. Scutellaria baicalensis regulates FFA metabolism to ameliorate NAFLD through the AMPK-mediated SREBP signaling pathway. J Nat Med (Epub ahead of print) (2018). WB Human
Lee YS et al. Thyroid Hormone Promotes β-Catenin Activation and Cell Proliferation in Colorectal Cancer. Horm Cancer (Epub ahead of print) (2018). WB Human
Cheng TM et al. Resveratrol induces sumoylated COX-2-dependent anti-proliferation in human prostate cancer LNCaP cells. Food Chem Toxicol (Epub ahead of print) (2017). WB Human
Hsieh CL et al. Reactive oxygen species-mediated switching expression of MMP-3 in stromal fibroblasts and cancer cells during prostate cancer progression. Sci Rep 7 (1):9065 (2017). WB Human
Tsai TH et al. Protective Effect of Caffeic Acid Derivatives on tert-Butyl Hydroperoxide-Induced Oxidative Hepato-Toxicity and Mitochondrial Dysfunction in HepG2 Cells. Molecules 22 (5) (2017). WB Human
Nickel S et al. Expression and Activity of Breast Cancer Resistance Protein (BCRP/ABCG2) in Human Distal Lung Epithelial Cells In Vitro. Pharm Res (Epub ahead of print) (2017). WB Human
Chao W et al. 3,4-Dihydroxybenzalactone Suppresses Human Non-Small Cell Lung Carcinoma Cells Metastasis via Suppression of Epithelial to Mesenchymal Transition, ROS-Mediated PI3K/AKT/MAPK/MMP and NFκB Signaling Pathways. Molecules 22 (4) (2017). WB Human
Zanini IM et al. Human Cactin interacts with DHX8 and SRRM2 to assure efficient pre-mRNA splicing and sister chromatid cohesion. J Cell Sci (Epub ahead of print) (2017). WB Human
Choi JH et al. Characterization of MUDENG, a novel anti-apoptotic protein. Oncogenesis 5:e221 (2016). WB Human
Santoro A et al. SERPINE2 Inhibits IL-1α-Induced MMP-13 Expression in Human Chondrocytes: Involvement of ERK/NF-κB/AP-1 Pathways. PLoS One 10 (8):e0135979 (2015). WB Human
Mandal SM et al. Amphotericin B and anidulafungin directly interact with DNA and induce oxidative damage in the mammalian genome. Mol Biosyst 11 (9):2551-9 (2015). WB Human
Lee JC et al. Andrographolide exerts anti-hepatitis C virus activity by up-regulating haeme oxygenase-1 via the p38 MAPK/Nrf2 pathway in human hepatoma cells. Br J Pharmacol 171 (1):237-52 (2014). WB Human
Tseng CK et al. Aqueous extract of Gracilaria tenuistipitata suppresses LPS-induced NF-κB and MAPK activation in RAW 264.7 and rat peritoneal macrophages and exerts hepatoprotective effects on carbon tetrachloride-treated rat. PLoS One 9 (1):e86557 (2014). WB Rat
Sobolesky PM et al. Regulation of the tumor suppressor FOXO3 by the thromboxane-A2 receptors in urothelial cancer. PLoS One 9 (9):e107530 (2014).
Chang YL et al. Regulation of estrogen receptor α function in oral squamous cell carcinoma cells by FAK signaling. Endocr Relat Cancer (2014).
Li HC et al. Nuclear export and import of human hepatitis B virus capsid protein and particles. PLoS Pathog 6 (10):e1001162 (2010). Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensLMNB14001P20700, B2R6J6150340, 169500NM_001198557NP_00118548643.03
Homo sapiensLMNB14001P20700, B2R6J6150340, 169500NM_005573NP_00556466.42

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusLmnb116906P14733NM_010721NP_03485166.8
Rattus norvegicusLmnb1116685P70615NM_053905NP_44635766.62
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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