MAD2L1 antibody [C2C3], C-term

Host / ClonalityRabbit Polyclonal
ApplicationsICC/IF, IHC-P, IP, WB
Species reactivityHuman, Rat
( 1 )

Cat No:

GTX104680

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Application Information: MAD2L1 antibody [C2C3], C-term

Catalog Number

GTX104680

Product Name

MAD2L1 antibody [C2C3], C-term

See all MAD2L1 products

Applications

ICC/IF, IHC-P, IP, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:1000*
IHC (Formalin-fixed paraffin-embedded sections)	1:100-1:1000*
Immunoprecipitation	1:100-1:500*	Y
Western Blot (WB)	1:500-1:3000*

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

24 kDa (note)

Positive Controls

293T , NCIN87 , U2OS , HepG2 , Molt4 , Raji

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: MAD2L1 antibody [C2C3], C-term

Full Name	MAD2 mitotic arrest deficient-like 1 (yeast)
Background	MAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq]
Synonyms	HSMAD2 antibody, MAD2 antibody, MAD2L1 antibody, "MAD2 (mitotic arrest deficient, yeast, homolog)-like 1 antibody", MAD2-like protein 1 antibody, mitotic spindle assembly checkpoint protein MAD2A antibody, "mitotic arrest deficient, yeast, homolog-like 1 antibody", mitotic arrest deficient 2-like protein 1 antibody, MAD2 mitotic arrest deficient-like 1 (yeast) antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MAD2L1. The exact sequence is proprietary.
Antigen Species	Human
Species Reactivity	Human, Rat
Predicted Cross Reactivity species Predicted cross-reactivity: Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive. Please note that we are only able to guarantee products to work in applications and species in which they have been tested.	Rhesus Monkey, Bovine
Predict Reactivity Note	Rhesus Monkey (100%), Bovine (100%)
Conjugation	Unconjugated

Storage Conditions: MAD2L1 antibody [C2C3], C-term

Storage Buffer	1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Cancer > Cell cycle > Cell division Cancer > Type of cancer > Breast > Ductal Cell Biology > Cell cycle More Hide

MAD2L1 antibody [C2C3], C-term validated data

GTX104680 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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GTX104680 IHC-P Image

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.

Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

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GTX104680 ICC/IF Image

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.

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GTX104680 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(GTX104680) antibody at 1:500 dilution.
Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Top

GTX104680 WB Image

Sample (30 µg whole cell lysate)
A:Hep G2 (GTX27900)
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
12% SDS PAGE
GTX104680 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX104680 IHC-P Image

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft .
MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.

Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Top

GTX104680 IP Image

Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 μg of MAD2L1 antibody [C2C3], C-term (GTX104680).
Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Top

GTX104680 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX104680 IHC-P Image

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.

Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Top

GTX104680 ICC/IF Image

Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.

Top

GTX104680 IHC-P Image

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(GTX104680) antibody at 1:500 dilution.
Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Top

GTX104680 WB Image

Sample (30 µg whole cell lysate)
A:Hep G2 (GTX27900)
B:MOLT4 (GTX27912)
C:Raji (GTX27908)
12% SDS PAGE
GTX104680 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX104680 IHC-P Image

MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded human A549 xenograft .
MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.

Antigen Retrieval: TrilogyTM (EDTA based) buffer, 15min

Top

GTX104680 IP Image

Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 μg of MAD2L1 antibody [C2C3], C-term (GTX104680).
Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Top

Reference for MAD2L1 antibody [C2C3], C-term

Application References	Application	Reactivity
Chen Y et al. The spindle checkpoint protein MAD1 regulates the expression of E-cadherin and prevents cell migration. Oncol Rep 27 (2):487-91 (2012).	IP	Human

Target Bioinfo/ Predicted Isoform Cross Reactivity
Species	Gene name	Gene ID	SwissProt	OMIM	Isoform (mRNA)	Protein Accession#	MW
Homo sapiens	MAD2L1	4085	Q548X9, Q13257	601467	NM_002358	NP_002349	23.51

*MSDS

MSDS-Glycerol.pdf

MSDS-PBS.pdf

MSDS-Thimerosal.pdf

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MAD2L1 antibody [C2C3], C-term

MAD2L1 antibody [C2C3], C-term

Application Information: MAD2L1 antibody [C2C3], C-term

Specifications: MAD2L1 antibody [C2C3], C-term

Storage Conditions: MAD2L1 antibody [C2C3], C-term