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  • MAD2L1 antibody [C2C3], C-term

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1 .GTX104680 MAD2L1 antibody [C2C3], C-term

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MAD2L1 antibody [C2C3], C-term

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  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, IP, WB
  • Species reactivityHuman, Rat
  • Publication  ( 1 )
Cat No: GTX104680
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680
  • Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX104680
  • Anti-MAD2L1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104680
  • Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX104680
  • Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680
  • Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX104680
  • Anti-MAD2L1 antibody [C2C3], C-term used in Immunoprecipitation. GTX104680
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  • Protocol & MSDS

  • Application Information: MAD2L1 antibody [C2C3], C-term

Catalog Number GTX104680
☆☆☆☆☆
( 0 )
Publication ( 1 )
Product Name MAD2L1 antibody [C2C3], C-term
See all MAD2L1 products
ApplicationsICC/IF, IHC-P, IP, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:100-1:500* Y
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size24 kDa (note)
Positive Controls293T , NCIN87 , U2OS , HepG2 , Molt4 , Raji
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: MAD2L1 antibody [C2C3], C-term

Full NameMAD2 mitotic arrest deficient-like 1 (yeast)
BackgroundMAD2L1 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is related to the MAD2L2 gene located on chromosome 1. A MAD2 pseudogene has been mapped to chromosome 14. [provided by RefSeq]
SynonymsHSMAD2 antibody, MAD2 antibody, MAD2L1 antibody, "MAD2 (mitotic arrest deficient, yeast, homolog)-like 1 antibody", MAD2-like protein 1 antibody, mitotic spindle assembly checkpoint protein MAD2A antibody, "mitotic arrest deficient, yeast, homolog-like 1 antibody", mitotic arrest deficient 2-like protein 1 antibody, MAD2 mitotic arrest deficient-like 1 (yeast) antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenCarrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MAD2L1. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
 		Rhesus Monkey, Bovine
Predict Reactivity NoteRhesus Monkey (100%), Bovine (100%)
ConjugationUnconjugated

  • Storage Conditions: MAD2L1 antibody [C2C3], C-term

Storage Buffer1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Cell cycle > Cell division
  • Cancer > Type of cancer > Breast > Ductal
  • Cell Biology > Cell cycle
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MAD2L1 antibody [C2C3], C-term validated data
  • Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680
    • GTX104680 WB Image  Knockout/Knockdown
      Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX104680
    • GTX104680 IHC-P Image
      MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis.
      Sample: Paraffin-embedded rat fore brain.
      MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104680
    • GTX104680 ICC/IF Image
      Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX104680
    • GTX104680 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(GTX104680) antibody at 1:500 dilution.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680
    • GTX104680 WB Image
      Sample (30 µg whole cell lysate)
      A:Hep G2 (GTX27900)
      B:MOLT4 (GTX27912)
      C:Raji (GTX27908)
      12% SDS PAGE
      GTX104680 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX104680
    • GTX104680 IHC-P Image
      MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis.
      Sample: Paraffin-embedded human A549 xenograft .
      MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in Immunoprecipitation. GTX104680
    • GTX104680 IP Image
      Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 μg of MAD2L1 antibody [C2C3], C-term (GTX104680).
      Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680
    • GTX104680 WB Image  Knockout/Knockdown
      Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX104680
    • GTX104680 IHC-P Image
      MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at nucleus on rat fore brain by immunohistochemical analysis.
      Sample: Paraffin-embedded rat fore brain.
      MAD2L1 antibody [C2C3], C-term (GTX104680) dilution: 1:500.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104680
    • GTX104680 ICC/IF Image
      Immunofluorescence analysis of human osteosarcoma cell line U2OS, using MAD2L1(GTX104680) antibody at 1:1000 dilution.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX104680
    • GTX104680 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Mad2L1(GTX104680) antibody at 1:500 dilution.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in Western Blot (WB). GTX104680
    • GTX104680 WB Image
      Sample (30 µg whole cell lysate)
      A:Hep G2 (GTX27900)
      B:MOLT4 (GTX27912)
      C:Raji (GTX27908)
      12% SDS PAGE
      GTX104680 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX104680
    • GTX104680 IHC-P Image
      MAD2L1 antibody [C2C3], C-term detects MAD2L1 protein at cytoplasm and nucleus in human A549 xenograft by immunohistochemical analysis.
      Sample: Paraffin-embedded human A549 xenograft .
      MAD2L1 antibody [C2C3], C-term (GTX104680) diluted at 1:250.
      Top
  • Anti-MAD2L1 antibody [C2C3], C-term used in Immunoprecipitation. GTX104680
    • GTX104680 IP Image
      Immunoprecipitation of MAD2L1 protein from 293T whole cell extracts using 5 μg of MAD2L1 antibody [C2C3], C-term (GTX104680).
      Western blot analysis was performed using MAD2L1 antibody [C2C3], C-term (GTX104680).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
Back to Top
  • Reference for MAD2L1 antibody [C2C3], C-term
Application References Application Reactivity
Chen Y et al. The spindle checkpoint protein MAD1 regulates the expression of E-cadherin and prevents cell migration. Oncol Rep 27 (2):487-91 (2012). IP Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensMAD2L14085Q548X9, Q13257601467NM_002358NP_00234923.51
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf

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