MBD2 (isoform 1) antibody, Internal
|Applications||IHC, IHC-P, WB|
|Application Note||Approx 48-50kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Biol. 2006 Oct;26(19):7224-35.; PMID: 16980624). Recommended concentration: 0.3-1µg/ml. In paraffin embedded Human Colon shows nuclear stianing of enterocytes. Recommended concentration, 2-4µg/ml. This antibody is expected to resognize isoform 1 (NP_003918.1) only.|
|Positive Controls||The peptide used to product this antibody is available for purchase (Catalog Number:GTX89182-PEP).|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.|
|Full Name||methyl-CpG binding domain protein 2|
|Product Description||Goat polyclonal antibody to MBD2 (isoform 1)|
|Background||Binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. Binds hemi-methylated DNA as well. Recruits histone deacetylases and DNA methyltransferases. Acts as transcriptional repressor and plays a role in gene silencing. Isoform 1 may enhance the activation of some unmethylated cAMP-responsive promoters. Reports about DNA demethylase activity of isoform 2 are contradictory. |
|Synonyms||DKFZp586O0821, methyl-CpG binding domain protein 2, MBD2 (isoform1), MBD2, NY-CO-41, MBD2 (isoform 1), methylCpG binding domain protein 2, NYCO41, methylCpG binding domain protein2, DMTase, NY CO 41|
|Cellular Localization||Nuclear, in discrete foci. Detected at replication foci in late S phase. |
|Specificity||This antibody is specific for MBD2 (isoform 1).|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Rat, Dog|