MCF-7 (human breast; epithelial; pleural effusion adenocarcinoma) cell line slides
|Product Description||MCF-7 cell line slides (human: breast; epithelial; pleural effusion adenocarcinoma)|
|Background||MCF7 (breast; epithelial; pleural effusion adenocarcinoma)|
Human MCF7 cells were cultured in Ham's F12K medium and harvested at the log phase of growth. The MCF7 cells were fixed in 4% paraformaldehyde and arrayed on a 12-well (5 mm) adhesive coated slide, with each wellis surface specifically treated to enhance cellular attachment and to minimize background staining. Each well was loaded with approximately 7.5 x 103 MCF7 cells. A follow-up heat dehydration process was done to ensure the attachment of MCF7 cells and the stability of cellular proteins.
|Application Note||MCF7 cell slide is provided for immunocytochemical analysis of protein and protein-protein interaction. Before applying immunocytochemistry, 0.1% Triton X-100 pretreatment for 1 minute at room temperature is highly suggested. Optimal conditions should be determined by the end user.|
|Storage instruction||MCF7 cell slide can be stored for up to 6 months at 4°C under desiccate conditions.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|