|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||91 kDa (note)
|Positive Controls||293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||minichromosome maintenance complex component 3|
|Product Description||Rabbit polyclonal antibody to MCM3 (minichromosome maintenance complex component 3)|
|Background||The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with, and thus is acetlyated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. [provided by RefSeq]|
|Synonyms||HCC5 antibody, MGC1157 antibody, P1-MCM3 antibody, P1.h antibody, RLFB antibody, MCM3 antibody, RLF subunit beta antibody, MCM3 minichromosome maintenance deficient 3 antibody, minichromosome maintenance deficient 3 antibody, DNA replication factor MCM3 antibody, DNA polymerase alpha holoenzyme-associated protein P1 antibody, p102 antibody, hRlf beta subunit antibody, "replication licensing factor, beta subunit antibody", cervical cancer proto-oncogene 5 antibody, DNA replication licensing factor MCM3 antibody, minichromosome maintenance complex component 3 antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human MCM3. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (94%), Zebrafish (80%), Xenopus laevis (84%), Chicken (86%), Rhesus Monkey (99%), Bovine (97%)|