|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||97 kDa (note)
|Positive Controls||Jurkat , Raji , NCI-H929 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||minichromosome maintenance complex component 4|
|Product Description||Rabbit Polyclonal antibody to MCM4 (minichromosome maintenance complex component 4)|
|Background||The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq]|
|Synonyms||CDC21 antibody, CDC54 antibody, MGC33310 antibody, P1-CDC21 antibody, hCdc21 antibody, MCM4 antibody, CDC21 homolog antibody, homolog of S. pombe cell devision cycle 21 antibody, MCM4 minichromosome maintenance deficient 4 antibody, minichromosome maintenance deficient 4 antibody, DNA replication licensing factor MCM4 antibody, minichromosome maintenance complex component 4 antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human MCM4. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rat (95%), Zebrafish (88%), Rhesus Monkey (99%), Bovine (93%)|