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1 .GTX129217 MCM6 antibody

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MCM6 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman
Cat No: GTX129217
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-MCM6 antibody used in Western Blot (WB). GTX129217
  • Anti-MCM6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129217
  • Anti-MCM6 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX129217
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  • Datasheet
  • Bio-info
  • Protocol & MSDS
  • Application Information: MCM6 antibody

Catalog Number GTX129217
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Product Name MCM6 antibody
See all MCM6 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Positive Controls293T , A431 , HeLa , HepG2
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: MCM6 antibody

Full Nameminichromosome maintenance complex component 6
BackgroundThe protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of the complex by CDC2 kinase reduces the helicase activity, suggesting a role in the regulation of DNA replication. [provided by RefSeq]
SynonymsMCG40308 Antibody, Mis5 Antibody, minichromosome maintenance complex component 6 Antibody, P105MCM Antibody
Cellular LocalizationNucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human MCM6. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Rat, Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteMouse (97%), Rat (97%), Chicken (90%), Rhesus Monkey (100%), Bovine (97%)
ConjugationUnconjugated

  • Storage Conditions: MCM6 antibody

Storage Buffer1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cell Biology > Cell cycle
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MCM6 antibody validated data
  • Anti-MCM6 antibody used in Western Blot (WB). GTX129217
    • GTX129217 WB Image
      MCM6 antibody detects MCM6 protein by Western blot analysis.
      A. 30 µg 293T whole cell lysate/extract
      B. 30 µg A431 whole cell lysate/extract
      C. 30 µg HeLa whole cell lysate/extract
      D. 30 µg HepG2 whole cell lysate/extract
      7.5 % SDS-PAGE
      MCM6 antibody (GTX129217) dilution: 1:1000
      Top
  • Anti-MCM6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129217
    • GTX129217 ICC/IF Image
      MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis.
      Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: MCM6 protein stained by MCM6 antibody (GTX129217) diluted at 1:1000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-MCM6 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX129217
    • GTX129217 IHC-P Image
      MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human ovarian carcinoma.
      MCM6 antibody (GTX129217) dilution: 1:1000.
      Top
  • Anti-MCM6 antibody used in Western Blot (WB). GTX129217
    • GTX129217 WB Image
      MCM6 antibody detects MCM6 protein by Western blot analysis.
      A. 30 µg 293T whole cell lysate/extract
      B. 30 µg A431 whole cell lysate/extract
      C. 30 µg HeLa whole cell lysate/extract
      D. 30 µg HepG2 whole cell lysate/extract
      7.5 % SDS-PAGE
      MCM6 antibody (GTX129217) dilution: 1:1000
      Top
  • Anti-MCM6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129217
    • GTX129217 ICC/IF Image
      MCM6 antibody detects MCM6 protein at nucleus by immunofluorescent analysis.
      Sample: U2OS cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: MCM6 protein stained by MCM6 antibody (GTX129217) diluted at 1:1000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-MCM6 antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX129217
    • GTX129217 IHC-P Image
      MCM6 antibody detects MCM6 protein at nucleus on human ovarian carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human ovarian carcinoma.
      MCM6 antibody (GTX129217) dilution: 1:1000.
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensMCM64175B2R6H2, Q14566223100, 601806, 606255NM_005915NP_00590692.9

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusMcm617219Q542I2, P97311NP_00130062489.8
Mus musculusMcm617219Q542I2, P97311NM_008567NP_03259392.88
Rattus norvegicusMcm629685A9CMB8NM_017287NP_05898392.83
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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All products are for research use only—Not for use in diagnostic procedures.