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1 .GTX48661 MCP1 / CCL2 antibody

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MCP1 / CCL2 antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsELISA, WB
  • Species reactivityHuman
Cat No: GTX48661
Package Price Qty
50 μgUSD 319.00
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  • Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48661
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  • Datasheet
  • Application Information: MCP1 / CCL2 antibody

Catalog Number GTX48661
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Product Name MCP1 / CCL2 antibody
See all MCP1 / CCL2 products
ApplicationsELISA, WB
Application Note
Suggested dilutionReference
ELISA1:10,000*  
Western Blot (WB)1:1,000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationIgG fraction

  • Specifications: MCP1 / CCL2 antibody

Full Namechemokine (C-C motif) ligand 2
BackgroundMCP-1, or Monocyte chemoattractant protein 1, of the intercrine beta (chemokine CC) family, is a chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. It augments monocyte anti-tumor activity and has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. MCP-1 exists as a monomer or homodimer; in equilibrium. It binds to CCR2 and CCR4 and is tethered on endothelial cells by glycosaminoglycan (GAG) side chains of proteoglycans. Processing at the N-terminus can regulate receptor and target cell selectivity. Deletion of the N-terminal residue converts it from an activator of basophil to an eosinophil chemoattractant.
SynonymsMCAF Antibody , CCL2 Antibody , SCYA2 Antibody , GDCF2 Antibody , HC11 Antibody , HSMCR30 Antibody , SMC CF Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenThis IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human MCP-1 protein produced in E.coli.
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: MCP1 / CCL2 antibody

Storage Buffer0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Storage InstructionStore vial at 4° C for short-term use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Apoptosis > Anti-apoptosis
  • Cancer > Hypoxia
  • Cancer > Type of cancer > Head and Neck > Nasopharyngeal
  • Cancer > Type of cancer > Liver
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MCP1 / CCL2 antibody validated data
  • Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48661
    • GTX48661 WB Image
      Western Blot of Anti-human MCP-1 antibody shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 µg, 8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with GeneTex's Anti-Human MCP-1 antibody. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
      Top
  • Anti-MCP1 / CCL2 antibody used in Western Blot (WB). GTX48661
    • GTX48661 WB Image
      Western Blot of Anti-human MCP-1 antibody shows detection of recombinant human MCP-1 raised in E.coli. Recombinant (0.1 µg, 8.6 kDa) protein was loaded onto and resolved by SDS-PAGE, then transferred to nitrocellulose. The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with GeneTex's Anti-Human MCP-1 antibody. After washing, membrane was probed with secondary antibody Dylight 649 Conjugated Anti-Rabbit IgG (H&L) (Goat) Antibody diluted 1:20,000 in blocking buffer) for 30 min. at RT. Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
      Top
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All products are for research use only—Not for use in diagnostic procedures.