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MDA5 antibody [N2C1], Internal

Orthogonal
Anti-MDA5 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX103138
Anti-MDA5 antibody [N2C1], Internal used in Western Blot (WB). GTX103138

Cat. No. GTX103138

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MDA5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

Positive Control

THP-1 (100 ng/ml PMA treatment for 16 hr and 1000 ng/ml LPS treatment for 24 hr)

Predict Reactivity

Mouse, Rat, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MDA5. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11171867

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon induced with helicase C domain 1 , AGS7 , Hlcd , IDDM19 , MDA-5 , MDA5 , RLR-2 , SGMRT1

Cellular Localization

Cytoplasm , Nucleus (Potential)

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein that is upregulated in response to treatment with beta-interferon (IFNB) and a protein kinase C-activating compound, mezerein (MEZ). Irreversible reprogramming of melanomas can be achieved by treatment with both these agents; treatment with either agent alone only achieves reversible differentiation. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MDA5 antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX103138

GTX103138 IHC-P Image

MDA5 antibody [N2C1], Internal detects MDA5 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MDA5 antibody [N2C1], Internal (GTX103138) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MDA5 antibody [N2C1], Internal used in Western Blot (WB). GTX103138

GTX103138 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDA5 antibody [N2C1], Internal (GTX103138) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Xing J et al. J Virol 2012; 86 (7) : 3528-40 Herpes simplex virus 1 tegument protein US11 downmodulates the RLR signaling pathway via direct interaction with RIG-I and MDA-5.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169