|Applications||ICC/IF, IHC-P, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||55 kDa (note)
|Positive Controls||H1299 , HCT116 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 , 293T , mouse MDM2-transfected 293T|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||Mdm2 p53 binding protein homolog|
|Product Description||Rabbit Polyclonal antibody to MDM2 (Mdm2 p53 binding protein homolog)
|Background||This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues. [provided by RefSeq]|
|Synonyms||HDMX antibody, MGC5370 antibody, MGC71221 antibody, hdm2 antibody, MDM2 antibody, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein antibody, MDM2 variant FB30 antibody, double minute 2, human homolog of, p53-binding protein antibody, MDM2 variant FB28 antibody, E3 ubiquitin-protein ligase Mdm2 antibody, oncoprotein Mdm2 antibody, Mdm2 p53 binding protein homolog antibody
|Cellular Localization||Nucleus , nucleoplasm , Cytoplasm , nucleolus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human MDM2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Dog, Cat, Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Dog (91%), Cat (95%), Pig (93%), Rhesus Monkey (98%), Bovine (94%)|