|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||64 kDa (note)
|Positive Controls||293T , A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||malic enzyme 1, NADP(+)-dependent, cytosolic|
|Product Description||Rabbit Polyclonal antibody to ME1 (malic enzyme 1, NADP(+)-dependent, cytosolic)|
|Background||This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet. [provided by RefSeq]|
|Synonyms||HUMNDME antibody, MES antibody, ME1 antibody, pyruvic-malic carboxylase antibody, "Malic enzyme, cytoplasmic antibody", "malic enzyme 1, soluble antibody", NADP-dependent malic enzyme antibody, malate dehydrogenase antibody, NADP-ME antibody, "malic enzyme 1, NADP(+)-dependent, cytosolic antibody"|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human ME1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Chicken, Sheep, Bovine|
|Predict Reactivity Note||Chicken (80%), Sheep (92%), Bovine (91%)|