MEF2A antibody [C2C3], C-term
|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||55 kDa (note)
|Positive Controls||A431 , H1299 , HeLa , HepG2 , PC-12 , Rat-2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||myocyte enhancer factor 2A|
|Product Description||Rabbit Polyclonal antibody to MEF2A (myocyte enhancer factor 2A)|
|Background||The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.[supplied by OMIM]|
|Synonyms||ADCAD1 antibody, RSRFC4 antibody, RSRFC9 antibody, MEF2A antibody, serum response factor-like protein 1 antibody, myocyte-specific enhancer factor 2A antibody, "MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A) antibody", myocyte enhancer factor 2A antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the C-terminus region of human MEF2A. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Pig (84%), Rhesus Monkey (95%), Bovine (83%)|