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  • MEK1 (phospho Ser221) antibody

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1 .GTX50216 MEK1 (phospho Ser221) antibody

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MEK1 (phospho Ser221) antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
Cat No: GTX50216
Package Price Qty
100 μlUSD 319.00
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  • Anti-MEK1 (phospho Ser221) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50216
  • Anti-MEK1 (phospho Ser221) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX50216
  • Anti-MEK1 (phospho Ser221) antibody used in Western Blot (WB). GTX50216
  • Anti-MEK1 (phospho Ser221) antibody used in Western Blot (WB). GTX50216
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  • Datasheet

  • Application Information: MEK1 (phospho Ser221) antibody

Catalog Number GTX50216
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Product Name MEK1 (phospho Ser221) antibody
See all MEK1 products
ApplicationsICC/IF, IHC-P, WB
Application NoteSuggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Predicted Target Size45 (note)
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationAntibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

  • Specifications: MEK1 (phospho Ser221) antibody

Full Namemitogen-activated protein kinase kinase 1
BackgroundCatalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.
SynonymsMAPKK1, MEK1, MKK1, PRKMK1, MAP2K1, protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), ERK activator kinase 1, MEK 1, MAP kinase kinase 1, MAPK/ERK kinase 1, dual specificity mitogen-activated protein kinase kinase 1, MAPKK 1, mitogen-activated protein kinase kinase 1
HostRabbit
ClonalityPolyclonal
ImmunogenPeptide sequence around phosphorylation site of serine 221 (A-N-S(p)-F-V) derived from Human MEK1.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
ConjugationUnconjugated

  • Storage Conditions: MEK1 (phospho Ser221) antibody

Storage Buffersaline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Prostate cancer
  • Cell Biology > Cell adhesion > Regulation > Negative regulation
  • Disease Related > Cardiovascular > Heart > Heart disease
  • Stem Cell Development > Development > Morphogenesis
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MEK1 (phospho Ser221) antibody validated data
  • Anti-MEK1 (phospho Ser221) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50216
    • GTX50216 ICC/IF Image
      Immunofluorescence staining of methanol-fixed Hela cells using MEK1 (Phospho-Ser221) Antibody (GTX50216).
      Top
  • Anti-MEK1 (phospho Ser221) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX50216
    • GTX50216 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho-Ser221) Antibody (GTX50216) (left) or the same antibody preincubated with blocking peptide (right).
      Top
  • Anti-MEK1 (phospho Ser221) antibody used in Western Blot (WB). GTX50216
    • GTX50216 WB Image
      Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1 (Phospho-Ser221) Antibody (GTX50216).
      Top
  • Anti-MEK1 (phospho Ser221) antibody used in Western Blot (WB). GTX50216
    • GTX50216 WB Image
      Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) GTX50216.
      Top
  • Anti-MEK1 (phospho Ser221) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX50216
    • GTX50216 ICC/IF Image
      Immunofluorescence staining of methanol-fixed Hela cells using MEK1 (Phospho-Ser221) Antibody (GTX50216).
      Top
  • Anti-MEK1 (phospho Ser221) antibody used in IHC (Formalin-fixed paraffin-embedded sections). GTX50216
    • GTX50216 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1 (Phospho-Ser221) Antibody (GTX50216) (left) or the same antibody preincubated with blocking peptide (right).
      Top
  • Anti-MEK1 (phospho Ser221) antibody used in Western Blot (WB). GTX50216
    • GTX50216 WB Image
      Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1 (Phospho-Ser221) Antibody (GTX50216).
      Top
  • Anti-MEK1 (phospho Ser221) antibody used in Western Blot (WB). GTX50216
    • GTX50216 WB Image
      Western blot analysis of extracts from Hela cells untreated or treated with EGF using MEK1(Phospho-Ser221) GTX50216.
      Top
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