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1 .GTX107529 MEK3 antibody [N1C3]

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MEK3 antibody [N1C3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse
Cat No: GTX107529
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-MEK3 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX107529
  • Anti-MEK3 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX107529
  • Anti-MEK3 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX107529
  • Anti-MEK3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107529
  • Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529
  • Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529
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  • Datasheet
  • Bio-info
  • Protocol & MSDS
  • Application Information: MEK3 antibody [N1C3]

Catalog Number GTX107529
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Product Name MEK3 antibody [N1C3]
See all MEK3 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size39 kDa (note) IsoForm (2)
Positive Controls293T , A431 , H1299 , HeLa , HepG2 , Raji , mouse liver
Form SuppliedLiquid
Concentration0.66 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: MEK3 antibody [N1C3]

Full Namemitogen-activated protein kinase kinase 3
BackgroundThe protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq]
SynonymsMAPKK3 antibody, MEK3 antibody, MKK3 antibody, PRKMK3 antibody, MAP2K3 antibody, MAPK/ERK kinase 3 antibody, MEK 3 antibody, SAPK kinase 2 antibody, MAP kinase kinase 3 antibody, MAPKK 3 antibody, dual specificity mitogen-activated protein kinase kinase 3 antibody, stress-activated protein kinase kinase 2 antibody, mitogen-activated protein kinase kinase 3 antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human MEK3. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Xenopus laevis, Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteRat (97%), Xenopus laevis (80%), Chicken (90%), Rhesus Monkey (100%), Bovine (97%)
ConjugationUnconjugated

  • Storage Conditions: MEK3 antibody [N1C3]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
MEK3 antibody [N1C3] validated data
  • Anti-MEK3 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX107529
    • GTX107529 IHC-P Image
      MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      MEK3 antibody [N1C3] (GTX107529) diluted at 1:500.
      Top
  • Anti-MEK3 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX107529
    • GTX107529 IHC-P Image
      MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse colon by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse colon.
      MEK3 antibody [N1C3] (GTX107529) diluted at 1:500.
      Top
  • Anti-MEK3 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX107529
    • GTX107529 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MEK3(GTX107529) antibody at 1:500 dilution.
      Top
  • Anti-MEK3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107529
    • GTX107529 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using MEK3(GTX107529) antibody at 1:200 dilution.
      Top
  • Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529
    • GTX107529 WB Image
      Sample (30 ug of whole cell lysate)
      A: A431 (GTX27909)
      B: Raji
      10% SDS PAGE
      GTX107529 diluted at 1:1000
      Top
  • Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529
    • GTX107529 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse liver
      10% SDS PAGE
      GTX107529 diluted at 1:500
      Top
  • Anti-MEK3 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX107529
    • GTX107529 IHC-P Image
      MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse kidney by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse kidney.
      MEK3 antibody [N1C3] (GTX107529) diluted at 1:500.
      Top
  • Anti-MEK3 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX107529
    • GTX107529 IHC-P Image
      MEK3 antibody [N1C3] detects MEK3 protein at cytoplasm in mouse colon by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse colon.
      MEK3 antibody [N1C3] (GTX107529) diluted at 1:500.
      Top
  • Anti-MEK3 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX107529
    • GTX107529 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MEK3(GTX107529) antibody at 1:500 dilution.
      Top
  • Anti-MEK3 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107529
    • GTX107529 ICC/IF Image
      Immunofluorescence analysis of methanol-fixed HeLa, using MEK3(GTX107529) antibody at 1:200 dilution.
      Top
  • Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529
    • GTX107529 WB Image
      Sample (30 ug of whole cell lysate)
      A: A431 (GTX27909)
      B: Raji
      10% SDS PAGE
      GTX107529 diluted at 1:1000
      Top
  • Anti-MEK3 antibody [N1C3] used in Western Blot (WB). GTX107529
    • GTX107529 WB Image
      Sample (50 ug of whole cell lysate)
      A: mouse liver
      10% SDS PAGE
      GTX107529 diluted at 1:500
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensMAP2K35606P46734, Q6FI23602315BC032478AAH3247839.32
Homo sapiensMAP2K35606P46734, Q6FI23602315NM_145109NP_65973139.32

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Rattus norvegicusMap2k3303200B1H230NM_001100674NP_00109414439.32
Mus musculusMap2k326397Q5SWN9, O09110NM_008928NP_03295439.3
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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