MHC Class II H-2I-Ak/S antibody [OX-6]
|Applications||FACS, ICC/IF, IHC-Fr, IHC-P, WB|
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1/50-1/100*
| Western blot||Assay-dependent dilution
Flow cytometry/FACS : Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
IHC (Formalin-fixed paraffin-embedded sections) : This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. PLP fixation is recommended for optimal results.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|