|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||74 kDa (note)
|Positive Controls||PC-3 , U87-MG , SK-N-SH , mouse brain , *DU145|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase)|
|Product Description||Rabbit Polyclonal antibody to MMP2 (matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase))|
|Background||Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]|
|Synonyms||CLG4 antibody, CLG4A antibody, MMP-II antibody, MONA antibody, TBE-1 antibody, MMP2 antibody, 72 kDa gelatinase antibody, matrix metalloproteinase-2 antibody, collagenase type IV-A antibody, gelatinase A antibody, 72 kDa type IV collagenase antibody, matrix metalloproteinase-II antibody, neutrophil gelatinase antibody, MMP-2 antibody, "matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) antibody"|
|Cellular Localization||Secreted , extracellular space , extracellular matrix |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human MMP2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Xenopus laevis, Pig, Rabbit, Chicken, Sheep, Bovine|
|Predict Reactivity Note||Rat (96%), Xenopus laevis (81%), Pig (94%), Rabbit (96%), Chicken (87%), Sheep (95%), Bovine (94%)|