MSH6 antibody [C1C2], Internal
|Applications||EMSA, ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
| Electrophoretic mobility shift assay||Assay-dependent dilution
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||153 kDa (note)
|Positive Controls||HepG2 , Neuro2A , GL261|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||mutS homolog 6 (E. coli)|
|Product Description||Rabbit Polyclonal antibody to MSH6 (mutS homolog 6 (E. coli))|
|Background||This gene encodes a protein similar to the MutS protein. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides, prior to their repair. A highly conserved region of approximately 150 aa, called the Walker-A adenine nucleotide binding motif, exists in MutS homologs. The encoded protein of this gene combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer. [provided by RefSeq]|
|Synonyms||GTBP antibody, HNPCC5 antibody, HSAP antibody, MSH6 antibody, DNA mismatch repair protein Msh6 antibody, mutS-alpha 160 kDa subunit antibody, GTMBP antibody, sperm-associated protein antibody, p160 antibody, hMSH6 antibody, G/T mismatch-binding protein antibody, mutS homolog 6 (E. coli) antibody|
|Cellular Localization||Nucleus |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human MSH6. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Zebrafish|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rhesus Monkey, Bovine|
|Predict Reactivity Note||Rhesus Monkey (99%), Bovine (93%)|