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MSR1 antibody

Orthogonal
Anti-MSR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32729
Anti-MSR1 antibody used in Western Blot (WB). GTX32729

Cat. No. GTX32729

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all MSR1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:10 - 1:100
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 192-451 of human MSR1 (NP_619729.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887679

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

macrophage scavenger receptor 1 , CD204 , SCARA1 , SR-A , SR-AI , SR-AII , SR-AIII , SRA , phSR1 , phSR2

Background

This gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing of this gene. These receptors or isoforms are macrophage-specific trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. It has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-MSR1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32729

GTX32729 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX32729 MSR1 antibody.
Blue : DAPI

Anti-MSR1 antibody used in Western Blot (WB). GTX32729

GTX32729 WB Image

Orthogonal

WB analysis of THP-1 treated by LPS (1 μg/ml) at 37ºC for 8 hours and PMA/TPA (200nM) for 30 minutes after serum-starvation for 16-20 hours and mouse liver tissue lysate using GTX32729 MSR1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Iftakhar-E-Khuda I et al. Proc Natl Acad Sci U S A 2016; 113 (38) : 10643-8 Gene-expression profiling of different arms of lymphatic vasculature identifies candidates for manipulation of cell traffic.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399