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Catalog Number
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GTX104990
(
0
)
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Product Name
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MTHFD2 antibody [N1C3]
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| Applications | ICC/IF, IHC-P, IP, WB |
| Application Note |
| Suggested dilution | Reference |
| Immunocytochemistry/ Immunofluorescence (ICC/IF) | 1:100-1:1000* |
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| IHC (Formalin-fixed paraffin-embedded sections) | 1:100-1:1000* |
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| Immunoprecipitation | 1:100-1:500* |
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| Western Blot (WB) | 1:500-1:3000* |
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Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
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| Predicted Target Size | 38 kDa (note)
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| Positive Controls | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , *SK-N-DZ , *SK-N-SH |
| Form Supplied | Liquid |
| Concentration | 0.41
mg/ml
(Please refer to the vial label for the specific concentration) |
| Purification | Purified by antigen-affinity chromatography. |
| Full Name | methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase |
| Product Description | Rabbit Polyclonal antibody to MTHFD2 (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase) |
| Background | This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq] |
| Synonyms | NMDMC antibody, MTHFD2 antibody, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase antibody, "bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody", "methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase antibody" |
| Cellular Localization | Mitochondrion |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MTHFD2. The exact sequence is proprietary. |
| Antigen Species | Human |
| Species Reactivity | Human |
Predicted Cross Reactivity species  Predicted cross-reactivity:
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
| Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Bovine |
| Predict Reactivity Note | Mouse (95%), Rat (93%), Zebrafish (86%), Xenopus laevis (90%), Chicken (92%), Bovine (95%) |
| Conjugation | Unconjugated |