Find Antibodies, Antibody Supplier - GeneTex Inc.
United States
  • Please select
    • All categories
    • Primary antibodies
    • Secondary antibodies
    • Antibody panels
    • Small molecules
    • Proteins
    • Peptides
    • Lysates
    • Slides
    • Sera & plasma
    • Research reagents
    • Research kits
    • Assay kits
    • ELISA kits
    • ELISA pairs
    • Isotype controls
My Account My Cart
Please login Product Support| Company News| Contact Us| SiteMap
  • Primary Antibodies

  • Secondary Antibodies

  • Proteins & Peptides

  • Lysates & Slides

  • Serum & Reagents

  • Research Kits

  • Isotype Controls

  • Home
  • Primary Antibodies
  • Primary antibodies
  • MTHFD2 antibody [N1C3]

Related products
More Hide
Recently viewed products

1 .GTX104990 MTHFD2 antibody [N1C3]

GTX101435 beta Catenin antibody [N1N2-2], N-term
Anti-beta Catenin antibody [N1N2-2], N-term used in IHC-P. GTX101435
GTX122148 Histone H3 antibody
Anti-Histone H3 antibody used in IHC-P. GTX122148
GTX128508 mCherry antibody
Anti-mCherry antibody used in IHC-Wm. GTX128508
GTX629448 5-Methylcytosine / 5-mC antibody [GT4111]
Anti-5-Methylcytosine / 5-mC antibody [GT4111] used in Dot. GTX629448
GTX629765 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612]
Anti-5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] used in MeDIP. GTX629765
GTX629890 SQSTM1 / P62 antibody [GT1478]
Anti-SQSTM1 / P62 antibody [GT1478] used in WB. GTX629890
ECL kit RIPA Extraction Kit Protein Ladder

MTHFD2 antibody [N1C3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, IP, WB
  • Species reactivityHuman
  • ★★★★★   (  1  )
Cat No: GTX104990
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-MTHFD2 antibody [N1C3] used in Immunoprecipitation. GTX104990
  • Anti-MTHFD2 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104990
  • Anti-MTHFD2 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104990
  • Anti-MTHFD2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104990
  • Anti-MTHFD2 antibody [N1C3] used in Western Blot (WB). GTX104990
  • Anti-MTHFD2 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104990
LinkButton
  • Datasheet
  • Star Review (1)
    Reference (0)
  • Bio-info
  • Protocol & MSDS
  • Application Information: MTHFD2 antibody [N1C3]

Catalog Number GTX104990
★★★★★
( 1 )
Publication ( 0 )
Product Name MTHFD2 antibody [N1C3]
See all MTHFD2 products
ApplicationsICC/IF, IHC-P, IP, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:100-1:500*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size38 kDa (note)
Positive Controls293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , *SK-N-DZ , *SK-N-SH
Form SuppliedLiquid
Concentration0.41 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: MTHFD2 antibody [N1C3]

Full Namemethylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
BackgroundThis gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq]
SynonymsNMDMC antibody, MTHFD2 antibody, NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase antibody, "bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial antibody", "methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase antibody"
Cellular LocalizationMitochondrion
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human MTHFD2. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Bovine
Predict Reactivity NoteMouse (95%), Rat (93%), Zebrafish (86%), Xenopus laevis (90%), Chicken (92%), Bovine (95%)
ConjugationUnconjugated

  • Storage Conditions: MTHFD2 antibody [N1C3]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Lung > Other
  • Disease Related > Cardiovascular > Heart > Heart disease
More Hide
MTHFD2 antibody [N1C3] validated data
  • Anti-MTHFD2 antibody [N1C3] used in Immunoprecipitation. GTX104990
    • GTX104990 IP Image
      Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 μg of MTHFD2 antibody [N1C3] (GTX104990).
      Western blot analysis was performed using MTHFD2 antibody [N1C3] (GTX104990).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104990
    • GTX104990 IHC-P Image
      MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
      Sample: Paraffin-embedded human MCF7 xenograft.
      MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104990
    • GTX104990 IHC-P Image
      MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human breast carcinoma.
      MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104990
    • GTX104990 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MTHFD2(GTX104990) antibody at 1:200 dilution.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in Western Blot (WB). GTX104990
    • GTX104990 WB Image
      Sample (30 µg of whole cell lysate)
      A: 293T
      B: A431 (GTX27909)
      C: H1299
      D: HeLa
      E: HepG2 (GTX27900)
      F: Molt-4 (GTX27912)
      G: Raji
      10% SDS PAGE
      GTX104990 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104990
    • GTX104990 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MTHFD2(GTX104990) antibody at 1:500 dilution.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in Immunoprecipitation. GTX104990
    • GTX104990 IP Image
      Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 μg of MTHFD2 antibody [N1C3] (GTX104990).
      Western blot analysis was performed using MTHFD2 antibody [N1C3] (GTX104990).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104990
    • GTX104990 IHC-P Image
      MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis.
      Sample: Paraffin-embedded human MCF7 xenograft.
      MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104990
    • GTX104990 IHC-P Image
      MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human breast carcinoma.
      MTHFD2 antibody [N1C3] (GTX104990) diluted at 1:500.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104990
    • GTX104990 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MTHFD2(GTX104990) antibody at 1:200 dilution.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in Western Blot (WB). GTX104990
    • GTX104990 WB Image
      Sample (30 µg of whole cell lysate)
      A: 293T
      B: A431 (GTX27909)
      C: H1299
      D: HeLa
      E: HepG2 (GTX27900)
      F: Molt-4 (GTX27912)
      G: Raji
      10% SDS PAGE
      GTX104990 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-MTHFD2 antibody [N1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX104990
    • GTX104990 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MTHFD2(GTX104990) antibody at 1:500 dilution.
      Top
  • StarReview
  • MTHFD2 antibody [N1C3]

  • Rating : ★★★★★ ( Average 5.0 based on 1 users reviews)
  • Western Blot (WB)(1) :★★★★★
  • Anonymous StarReview submitted on 04 Dec 2014
  • Score : ★★★★★
  • Application Tested : WB
  • Sample Species : Human
  • Sample : SK-N-DZ cells, SK-N-SH cells
  • Amount used(ug/cells) : 20ug
  • Blocking conditions
  • Blocking Buffer : 5% milk
  • Incubation Time & Temperature : 25 (℃)/1hr
  • Primary antibody
  • Dilution : 1:1000( conc. of dilution )
  • Incubation Time & Temperature : 25 (℃)/2hr
  • Data Description
  • Lane1: SK-N-DZ cells Lane2: SK-N-SH cells
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensMTHFD210797Q7Z650, P13995604887NM_006636NP_00662737.9

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusMthfd217768P18155NM_008638NP_03266437.87
Rattus norvegicusMthfd2680308NM_001109398NP_00110286837.72
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
  • Product types

    Primary Antibodies Secondary Antibodies Proteins & Peptides Lysates & Slides Serum & Reagents Research Kits Isotype Controls

    Resources

    Literature Newsletters Protocols Pathways

  • Research Areas

    Cancer Cell Biology Neuroscience Metabolism Epigenetics Immunology Signal Transduction Infectious Disease Disease Related Stem Cell Development Zebrafish Epitope Tags

  • Company

    About Us News Rewards Program Recent Publications Contact Us Policy Guarantee Careers Sitemap

    Support

    Protocols Technical Tips FAQ

  • Distributors

    Custom Antibodies

    Member Account

    • Youtube Youtube
    • Facebook Facebook
    • Twitter Twitter
    • LinkedIn LinkedIn
    • Weibo Weibo
© 2013 GeneTex Inc. All rights reserved.
All products are for research use only—Not for use in diagnostic procedures.