|Background||Disruption of the mitochondrial transmembrane potential is one of the earliest intracellular events that occur following induction of apoptosis. The MitoLume? Apoptosis Detection Kit provides a simple, fluorescent-based method for distinguishing between healthy and apoptotic cells by detecting the changes in the mitochondrial transmembrane potential. The kit utilizes MitoLume?, a cationic dye that fluoresces differently in healthy vs apoptotic cells. In healthy cells, MitoLume accumulates and aggregates in the mitocondria, giving off a bright red fluorescence. In apoptotic cells, MitoLume cannot aggregate in the mitochondria due to the altered mitochondrial transmembrane potential, and thus it remains in the cytoplasm in its monomer form, fluorescing green. The fluorescent signals can be easily detected by fluorescence microscopy using a band-pass filter (detects FITC and rhodamine) or analyzed by flow cytometry using FITC channel for green monomers (Ex/Em = 488/530+ 30 nm) and (optional) PI channel for red aggregates (Em = 488/590+ 42 nm). |
‧ Detection method- Fluorecence microscopy and Fluorescent plate reader
‧ Sample type- Live cells
‧ Species reactivity- Mammalian
‧ Applications- Disruption of mitochondrial transmembrane potential is one of the earliest intracellular events that occur upon induction of apoptosis. The MitoLume Mitochondrial Apoptosis Detection Kit provides a simple and sensitive in vitro assay for detecting the mitochondrial changes in apoptosis. It is highly sensive and detects apoptosis in living cells.
Features and Benefits
‧ Simple one-step procedure; takes only 30 minutes
‧ Fast and convenient
‧ The assay utilizes a novel cationic dye (called MitoLume) that fluoresces differently in healthy versus apoptotic cells. In healthy cells MitoLume accumulates and aggregates in the mitochondria-fluorescing bright red. In apoptotic cells a shift in mitochondrial transmembrane potential prevents the dye from accumulating in the mitochondria. Instead it remains in the cytoplasm in its monomer form-fluorescing green.