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Goat Anti-Mouse IgG antibody (HRP)

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01
Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

Cat. No. GTX213111-01

Reference 354

Host

 Goat

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA, IHC

Reactivity

 Mouse
Package
1 ml ($149)
See all Mouse IgG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
IHC-P 1:100-1:1000
ELISA Assay dependent
IHC Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.25 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Highly purified whole mouse IgG

Target Isotype

IgG

Purification

Purified by antigen-affinity chromatography.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_10618076

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Goat antiMouse IgG HRP , Mouse IgG secondary antibody , antiMouse IgG antibody , anti-Mouse IgG antibody , Goat anti-Mouse IgG HRP , anti Mouse IgG antibody , Goat anti Mouse IgG HRP

Background

Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/ml in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.

DATA IMAGES

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 IHC-P Image

E2F1 antibody [16G7] detects E2F1 protein at nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
E2F1 antibody [16G7] (GTX70163) diluted at 1:500.
The signal was developed by Mouse IgG antibody (HRP) (GTX213111-01).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

Various whole cell extracts were separated by 12% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

Various amounts of denatured mouse IgG protein were separated by 12% SDS-PAGE, and the membrane was blotted with HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) diluted at 1:5000.

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

The data was published in the journal EMBO Rep in 2020. PMID: 31885126

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

The data was published in the journal EMBO Rep in 2020. PMID: 31885126

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

The data was published in the journal Mar Drugs in 2020. PMID: 32245246

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

The data was published in the journal EMBO Rep in 2020. PMID: 31885126

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

The data was published in the 2022 in Front Aging Neurosci. PMID: 35264943

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

The data was published in the 2022 in Front Aging Neurosci. PMID: 35264943

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

The data was published in the 2017 in Nat Commun. PMID: 28281525

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

The data was published in the 2017 in Sci Rep. PMID: 28387350

Goat Anti-Mouse IgG antibody (HRP). GTX213111-01

GTX213111-01 WB Image

The data was published in the 2017 in Nat Commun. PMID: 28281525

REFERENCE

Application Reference

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Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3) : e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
HJ Kim et al. Oncol Rep 2024; 51 (2) : 21 Caffeic acid phenethyl ester: Unveiling its potential as a potent apoptosis inducer for combating hypopharyngeal squamous cell carcinoma
Application : WB
Choo M et al. Acta Pharmacol Sin 2023; 44 (3) : 670-679 Involvement of cell shape and lipid metabolism in glioblastoma resistance to temozolomide.
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REVIEW

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Application Reference
Dodantenna N et al. Int J Mol Sci 2024; 25 (4) The African Swine Fever Virus Virulence Determinant DP96R Suppresses Type I IFN Production Targeting IRF3.
Hu WW et al. Bioeng Transl Med 2024; 9 (2):e10633 Development of a multifunctional bioreactor to evaluate the promotion effects of cyclic stretching and electrical stimulation on muscle differentiation.
Cheng CA et al. Adv Sci (Weinh) 2024; 11 (3):e2304926 Ultrasensitive and High-Resolution Protein Spatially Decoding Framework for Tumor Extracellular Vesicles.
HJ Kim et al. Oncol Rep 2024; 51 (2):21 Caffeic acid phenethyl ester: Unveiling its potential as a potent apoptosis inducer for combating hypopharyngeal squamous cell carcinoma
Application : WB
Choo M et al. Acta Pharmacol Sin 2023; 44 (3):670-679 Involvement of cell shape and lipid metabolism in glioblastoma resistance to temozolomide.
Yang S et al. BMB Rep 2023; 56 (12):651-656 Fatty acid oxidation regulates cellular senescence by modulating the autophagy-SIRT1 axis.
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Application : WB
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