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1 .GTX112734 N-Cadherin antibody [N1N3]

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N-Cadherin antibody [N1N3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
  • Publication  ( 4 )
Cat No: GTX112734
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
  • Anti-N-Cadherin antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX112734
  • Anti-N-Cadherin antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX112734
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
  • Anti-N-Cadherin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112734
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  • Application Information: N-Cadherin antibody [N1N3]

Catalog Number GTX112734
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( 0 )
Publication ( 4 )
Product Name N-Cadherin antibody [N1N3]
See all N-Cadherin products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size100 kDa (note) IsoForm (2)
Positive ControlsNT2D1 , U87-MG , SK-N-SH , IMR-32 , SK-N-AS , mouse brain , rat brain , PC-12 , Rat2
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: N-Cadherin antibody [N1N3]

Full Namecadherin 2, type 1, N-cadherin (neuronal)
BackgroundThis gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product. [provided by RefSeq]
Synonymsneural-cadherin antibody, neural cadherin antibody, calcium-dependent adhesion protein, neuronal antibody, cadherin-2 antibody, cadherin 2, type 1, N-cadherin (neuronal) antibody, cadherin 2, N-cadherin (neuronal) antibody, NCAD antibody, N-cadherin 1 antibody, CDw325 antibody, CDHN antibody, CDH2 antibody, CD325 antibody
Cellular LocalizationCell membrane
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human N-Cadherin. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Xenopus laevis, Dog, Chicken, Bovine
Predict Reactivity NoteXenopus laevis (82%), Dog (96%), Chicken (90%), Bovine (96%)
ConjugationUnconjugated

  • Storage Conditions: N-Cadherin antibody [N1N3]

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Head and Neck > Esophageal
  • Cancer > Type of cancer > Head and Neck > Laryngeal
  • Cancer > Type of cancer > Head and Neck > Pharyngeal
  • Cell Biology > Cell adhesion > Cell-cell adhesion > Calcium-dependent
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N-Cadherin antibody [N1N3] validated data
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis.
      A. 30 µg PC-12 whole cell extract
      B. 30 µg Rat2 whole cell extract
      5% SDS-PAGE
      N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX112734
    • GTX112734 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using N-Cadherin(GTX112734) antibody at 1:500 dilution.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX112734
    • GTX112734 IHC-P Image
      N-Cadherin antibody [N1N3] detects CDH2 protein at membrane on hepatoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human hepatoma.
      N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:500.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-cadherin antibody (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112734
    • GTX112734 ICC/IF Image
      N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane and cytoplasm by immunofluorescent analysis.
      Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: N-Cadherin protein stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      N-Cadherin antibody [N1N3] detects N-Cadherin protein by western blot analysis.
      A. 30 µg PC-12 whole cell extract
      B. 30 µg Rat2 whole cell extract
      5% SDS-PAGE
      N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX112734
    • GTX112734 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using N-Cadherin(GTX112734) antibody at 1:500 dilution.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX112734
    • GTX112734 IHC-P Image
      N-Cadherin antibody [N1N3] detects CDH2 protein at membrane on hepatoma by immunohistochemical analysis.
      Sample: Paraffin-embedded human hepatoma.
      N-Cadherin antibody [N1N3] (GTX112734) dilution: 1:500.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      Whole cell extract (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Western Blot (WB). GTX112734
    • GTX112734 WB Image
      Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with N-cadherin antibody (GTX112734) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-N-Cadherin antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112734
    • GTX112734 ICC/IF Image
      N-Cadherin antibody [N1N3] detects N-Cadherin protein at cell membrane and cytoplasm by immunofluorescent analysis.
      Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: N-Cadherin protein stained by N-Cadherin antibody [N1N3] (GTX112734) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody (GTX631836) diluted at 1:200.
      Blue: Hoechst 33342 staining.
      Top
Back to Top
  • Reference for N-Cadherin antibody [N1N3]
Application References Application Reactivity
Ling HH et al. Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer. Exp Cell Res (2016). WB Human
Jian MY et al. N-cadherin coordinates AMP kinase-mediated lung vascular repair. Am J Physiol Lung Cell Mol Physiol 310 (1):L71-85 (2016). WB Rat
Lupia A et al. CD63 tetraspanin is a negative driver of epithelial-to-mesenchymal transition in human melanoma cells. J Invest Dermatol 134 (12):2947-56 (2014). WB Human
Hsu TH et al. Involvement of RARRES3 in the regulation of Wnt proteins acylation and signaling activities in human breast cancer cells. Cell Death Differ (2014). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensCDH21000P19022, B0YIY6114020NP_00129510597.06
Homo sapiensCDH21000P19022, B0YIY6114020NM_001792NP_00178399.83

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusCdh212558Q3UIC2, Q6GU11NM_007664NP_03169099.81
Rattus norvegicusCdh283501Q9Z1Y3NM_031333NP_11262399.83
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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