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NAT5 antibody

Anti-NAT5 antibody used in Western Blot (WB). GTX32736
Anti-NAT5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32736
Anti-NAT5 antibody used in Immunoprecipitation (IP). GTX32736
Anti-NAT5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32736
Anti-NAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32736

Cat. No. GTX32736

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all NAT5 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
Not tested in other applications.

Calculated MW

20 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 49-178 of human NAA20 (NP_057184.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

N(alpha)-acetyltransferase 20, NatB catalytic subunit , NAT3 , NAT3P , NAT5 , NAT5P , dJ1002M8.1

Cellular Localization

Cytoplasm,Nucleus

Background

NAT5 is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminal acetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 [PubMed 18570629]).[supplied by OMIM, Apr 2009]

Database

Research Area

DATA IMAGES

Anti-NAT5 antibody used in Western Blot (WB). GTX32736

GTX32736 WB Image

WB analysis of various sample lysates using GTX32736 NAT5 antibody. The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

Anti-NAT5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32736

GTX32736 IHC-P Image

IHC-P analysis of human oophoroma tissue using GTX32736 NAT5 antibody.
Dilution : 1:100

Anti-NAT5 antibody used in Immunoprecipitation (IP). GTX32736

GTX32736 IP Image

IP analysis of MCF-7 cell lysate using GTX32736 NAT5 antibody.
Antibody amount : 1μg / 200μg lysate
Dilution : 1:1000

Anti-NAT5 antibody used in IHC (Paraffin sections) (IHC-P). GTX32736

GTX32736 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX32736 NAT5 antibody.
Dilution : 1:100

Anti-NAT5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32736

GTX32736 ICC/IF Image

ICC/IF analysis of A549 cells using GTX32736 NAT5 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399