NBS1 antibody [1D7]
|Synonyms||NBS p95, nibrin, nibrin breakage syndrome, MRN, hNBS1, hNBS|
|Product Description||Mouse monoclonal [1D7] NBS1|
|Background||Nijmegen breakage syndrome (NBS, nibrin) is an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The 1C3 antibody recognizes the 95 kDa NBS protein which contains a fork FHA head-associated domain that is adjacent to BRCT, a breast cancer C terminal domain, that has been implicated in protein-protein interactions. NBS is member of the Mre11/Rad50, p400 double-strand break repair complex involved in double-strand break repair and cell-cycle checkpoint functions.|
|Immunogen||The complete coding region of the human p95/NBS1 expressed in E. coli.|
|Species Reactivity||Human, Mouse, Rat|
|Applications||ICC/IF, IP, WB|
|Application Note||Recommended Starting Dilutions:|
For IP: Use at an assay dependent dilution.
For ICC/IF: Use at a dilution of 1:100
For WB: Use at a concentration of 1 - 2 µg/ml.
Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher.
|Positive Controls||MCF7, HeLa (NE), Raji|
|Predicted Target Size||95|
|Purification||Protein G Affinity Purified|
|Purification Note||From ascitic fluid|
|Storage Buffer||Phosphate-buffered saline, pH 7.4, containing no preservatives |
|Storage Instruction||Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.|
|Notes||For in vitro research use only. Not intended for any diagnostic or therapeutic purpose. Not suitable for human or animal consumption.|