GeneTex
  • Country / Location Selection

United States (US)

NBS1 antibody [1C3]

Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222
Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222
Anti-NBS1 antibody [1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70222
Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222

Cat. No. GTX70222

Host

Mouse

Clonality

Monoclonal

Clone Name

1C3

Isotype

IgG1

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

85 kDa. ( Note )

Positive Control

293T , MCF7 , HeLa , Raji

PROPERTIES

Form

Liquid

Buffer

1XPBS (pH7), 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The complete coding region of the human p95/NBS1 expressed in E. coli.

Purification

Protein G purified

Conjugation

Unconjugated

RRID

AB_372443

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nibrin , AT-V1 , AT-V2 , ATV , NBS , NBS1 , P95

Cellular Localization

Nucleus

Background

Mutations in this gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222

GTX70222 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1C3] (GTX70222) diluted at 1:500.

Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222

GTX70222 WB Image

Sample (50 ug of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX70222 diluted at 1:1000

Anti-NBS1 antibody [1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70222

GTX70222 ICC/IF Image

Immunofluorescence analysis of HeLa, using NBS1(GTX70222) antibody at 1:100 dilution.

Anti-NBS1 antibody [1C3] used in Western Blot (WB). GTX70222

GTX70222 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NBS1 antibody [1C3] (GTX70222) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

REVIEW

NBS1 antibody [1C3] Cat. No. GTX70222

Rating ( Average 5 based on 2 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal J Virol in 2017.PMID: 28855246
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal BMC Biol in 2015. PMID: 25857663
Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 399