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  • Nucleolin antibody, C-term

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1 .GTX47363 Nucleolin antibody, C-term

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Nucleolin antibody, C-term


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman
Cat No: GTX47363
Package Price Qty
100 μgUSD 319.00
Add to cart
 
  • Anti-Nucleolin antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47363
  • Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363
  • Anti-Nucleolin antibody, C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX47363
  • Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363
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  • Datasheet

  • Application Information: Nucleolin antibody, C-term

Catalog Number GTX47363
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Product Name Nucleolin antibody, C-term
See all Nucleolin products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)Assay-dependent dilution  
IHC (Formalin-fixed paraffin-embedded sections)2-10 ug/ml*  
Western Blot (WB)0.2-2.5 ug/ml*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size77 KDa (note)
Positive ControlsDaudi , NT-2
Form SuppliedLiquid
Concentration0.5-1 mg/ml
PurificationProtein A purified

  • Specifications: Nucleolin antibody, C-term

Full Namenucleolin
BackgroundNucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
SynonymsNCL Antibody , C23 Antibody
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenA synthetic peptide corresponding to a C-terminal region of Human NCL
Antigen SpeciesHuman
Species ReactivityHuman
Predict Reactivity NoteCow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%
ConjugationUnconjugated

  • Storage Conditions: Nucleolin antibody, C-term

Storage Buffer1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Storage InstructionKeep as concentrated solution. For short-term storage, store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Angiogenesis
  • Disease Related > Cardiovascular > Angiogenesis
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Nucleolin antibody, C-term validated data
  • Anti-Nucleolin antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47363
    • GTX47363 ICC/IF Image
      ICC/IF analysis of NT-2 cells using GTX47363 NCL antibody at 1:500.
      Red: NCL
      Blue:DAPI
      Top
  • Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363
    • GTX47363 WB Image
      WB analysis of NT-2 cells using GTX47363 NCL antibody at 2μg/ml.
      Top
  • Anti-Nucleolin antibody, C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX47363
    • GTX47363 IHC-P Image
      IHC-P analysis of human kidney tissue using GTX47363 NCL antibody at 4.0-8.0μg/ml.
      Top
  • Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363
    • GTX47363 WB Image
      WB analysis of Daudi cells using GTX47363 NCL antibody at 1.25μg/ml.
      Lane A: marker
      Lane B: Daudi cells
      Top
  • Anti-Nucleolin antibody, C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX47363
    • GTX47363 ICC/IF Image
      ICC/IF analysis of NT-2 cells using GTX47363 NCL antibody at 1:500.
      Red: NCL
      Blue:DAPI
      Top
  • Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363
    • GTX47363 WB Image
      WB analysis of NT-2 cells using GTX47363 NCL antibody at 2μg/ml.
      Top
  • Anti-Nucleolin antibody, C-term used in IHC (Formalin-fixed paraffin-embedded sections). GTX47363
    • GTX47363 IHC-P Image
      IHC-P analysis of human kidney tissue using GTX47363 NCL antibody at 4.0-8.0μg/ml.
      Top
  • Anti-Nucleolin antibody, C-term used in Western Blot (WB). GTX47363
    • GTX47363 WB Image
      WB analysis of Daudi cells using GTX47363 NCL antibody at 1.25μg/ml.
      Lane A: marker
      Lane B: Daudi cells
      Top
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