|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||52 kDa (note)
|Positive Controls||Raji , HepG2 , HepG2 whole cell extract (untreated) , HepG2 whole cell extract (3 µM Thapsigargin treatment for 12 hr) , HepG2 whole cell extract (3 µM Thapsigargin treatment for 24 hr) , Huh-7 (untreated) , Huh-7 (3 µM Thapsigargin treatment for 12 hr) , Huh-7 (3 µM Thapsigargin treatment for 24 hr)|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||calcium binding and coiled-coil domain 2|
|Product Description||Rabbit Polyclonal antibody to CALCOCO2 (calcium binding and coiled-coil domain 2)|
|Background||The protein encoded by this gene is a subunit of nuclear domain 10 (ND10) bodies. ND10 bodies are nuclear domains appearing immunohistochemically as ten dots per nucleus. They are believed to be associated with the nuclear matrix on the basis of their resistance to nuclease digestion and salt extraction. ND10 proteins are removed from the nucleus by herpes simplex virus-1 infection and may have a role in viral life cycles. [provided by RefSeq]|
|Synonyms||MGC17318 antibody, NDP52 antibody, CALCOCO2 antibody, nuclear domain 10 protein NDP52 antibody, nuclear dot protein 52 antibody, antigen nuclear dot 52 kDa protein antibody, nuclear domain 10 protein 52 antibody, calcium-binding and coiled-coil domain-containing protein 2 antibody, calcium binding and coiled-coil domain 2 antibody|
|Cellular Localization||Cytoplasm , perinuclear region , Golgi apparatus , cytoskeleton |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human NDP52. The exact sequence is proprietary.|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Predict Reactivity Note||Chimpanzee (97%)|