|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||53 kDa (note)
|Positive Controls||293T , A431 , Jurkat , Raji , mouse brain , rat brain|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase)|
|Product Description||Rabbit Polyclonal antibody to NDUFS2 (NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase))|
|Background||Mitochondrial complex I (NADH-ubiquinone reductase; EC 22.214.171.124) is the first multimeric complex of the respiratory chain that catalyzes the NADH oxidation with concomitant ubiquinone reduction and proton ejection out of the mitochondria. Mammalian mitochondrial complex I is an assembly of at least 43 different subunits. Seven of the subunits are encoded by the mitochondrial genome; the remainder are the products of nuclear genes. The iron-sulfur protein (IP) fraction of complex I is made up of 7 subunits, including NDUFS2. See NDUFS1 (MIM 157655).[supplied by OMIM]|
|Synonyms||CI-49 antibody, NDUFS2 antibody, complex 1, mitochondrial respiratory chain, 49-KD subunit antibody, NADH dehydrogenase [ubiquinone] iron-sulfur protein 2, mitochondrial antibody, NADH-ubiquinone oxidoreductase 49 kDa subunit antibody, complex I 49kDa subunit antibody, complex I-49kD antibody, NADH-ubiquinone oxidoreductase NDUFS2 subunit antibody, CI-49kD antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 2, 49kDa (NADH-coenzyme Q reductase) antibody|
|Cellular Localization||Mitochondrion inner membrane; Peripheral membrane protein; Matrix side |
|Immunogen||Recombinant protein encompassing a sequence within the center region of human NDUFS2. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Zebrafish, Xenopus laevis, Rhesus Monkey, Chimpanzee, Bovine|
|Predict Reactivity Note||Zebrafish (84%), Xenopus laevis (90%), Rhesus Monkey (98%), Chimpanzee (100%), Bovine (94%)|