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  • NEK4 antibody [N2C1], Internal

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1 .GTX102826 NEK4 antibody [N2C1], Internal

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NEK4 antibody [N2C1], Internal


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman
Cat No: GTX102826
Package Price Qty
25 μlUSD 169.00
100 μlUSD 319.00
Add to cart
 
  • Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826
  • Anti-NEK4 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102826
  • Anti-NEK4 antibody [N2C1], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102826
  • Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826
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  • Application Information: NEK4 antibody [N2C1], Internal

Catalog Number GTX102826
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Product Name NEK4 antibody [N2C1], Internal
See all NEK4 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size95 kDa (note) IsoForm (3)
Positive ControlsA431 , H1299 , U87-MG , SK-N-SH , IMR32 , SK-N-AS
Form SuppliedLiquid
Concentration0.37 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: NEK4 antibody [N2C1], Internal

Full NameNIMA (never in mitosis gene a)-related kinase 4
SynonymsMGC33171 antibody, NRK2 antibody, STK2 antibody, pp12301 antibody, NEK4 antibody, serine/threonine protein kinase-2 antibody, serine/threonine-protein kinase Nek4 antibody, serine/threonine-protein kinase 2 antibody, nimA-related protein kinase 4 antibody, serine/threonine-protein kinase NRK2 antibody, never in mitosis A-related kinase 4 antibody, serine/threonine kinase 2 antibody, NIMA (never in mitosis gene a)-related kinase 4 antibody
Cellular LocalizationNucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human NEK4. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
ConjugationUnconjugated

  • Storage Conditions: NEK4 antibody [N2C1], Internal

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionStore as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Cell cycle > Cell division
  • Cell Biology > Cell cycle
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NEK4 antibody [N2C1], Internal validated data
  • Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826
    • GTX102826 WB Image
      Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-NEK4 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102826
    • GTX102826 ICC/IF Image
      NEK4 antibody [N2C1], Internal detects NEK4 protein at nucleus by confocal immunofluorescent analysis.
      Sample: HeLa cells were fixed in ice-cold MeOH for 15 min.
      Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:500.
      Blue: Hoechst 33343 staining.
      [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
      Top
  • Anti-NEK4 antibody [N2C1], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102826
    • GTX102826 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4(GTX102826) antibody at 1:100 dilution.
      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
      Top
  • Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826
    • GTX102826 WB Image
      Sample (30 ug of whole cell lysate)
      A: A431 (GTX27909)
      B: H1299
      7.5% SDS PAGE
      GTX102826 diluted at 1:1000
      Top
  • Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826
    • GTX102826 WB Image
      Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-NEK4 antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102826
    • GTX102826 ICC/IF Image
      NEK4 antibody [N2C1], Internal detects NEK4 protein at nucleus by confocal immunofluorescent analysis.
      Sample: HeLa cells were fixed in ice-cold MeOH for 15 min.
      Green: NEK4 protein stained by NEK4 antibody [N2C1], Internal (GTX102826) diluted at 1:500.
      Blue: Hoechst 33343 staining.
      [Images captured by Olympus FV10i Confocal Laser Scanning Microscope]
      Top
  • Anti-NEK4 antibody [N2C1], Internal used in IHC (Formalin-fixed paraffin-embedded sections). GTX102826
    • GTX102826 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using NEK4(GTX102826) antibody at 1:100 dilution.
      Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
      Top
  • Anti-NEK4 antibody [N2C1], Internal used in Western Blot (WB). GTX102826
    • GTX102826 WB Image
      Sample (30 ug of whole cell lysate)
      A: A431 (GTX27909)
      B: H1299
      7.5% SDS PAGE
      GTX102826 diluted at 1:1000
      Top
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Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensNEK46787P51957, A5YM70601959NP_00133534385.27
Homo sapiensNEK46787P51957, A5YM70601959NM_001193533NP_00118046284.36
Homo sapiensNEK46787P51957, A5YM70601959NM_003157NP_00314894.62
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf

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