|Applications||ICC/IF, IHC-P, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||37 kDa (note)
|Positive Controls||A431 , HeLa , HepG2 , NIH-3T3 , JC , BCL-1 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||nuclear transcription factor Y, alpha|
|Product Description||Rabbit Polyclonal antibody to NFYA (nuclear transcription factor Y subunit alpha)|
|Background||The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds to CCAAT motifs in the promoter regions in a variety of genes. Subunit A associates with a tight dimer composed of the B and C subunits, resulting in a trimer that binds to DNA with high specificity and affinity. The sequence specific interactions of the complex are made by the A subunit, suggesting a role as the regulatory subunit. In addition, there is evidence of post-transcriptional regulation in this gene product, either by protein degradation or control of translation. Further regulation is represented by alternative splicing in the glutamine-rich activation domain, with clear tissue-specific preferences for the two isoforms. [provided by RefSeq]|
|Synonyms||CBF-A antibody, CBF-B antibody, FLJ11236 antibody, HAP2 antibody, NF-YA antibody, NFYA antibody, CAAT box DNA-binding protein subunit A antibody, CCAAT-binding transcription factor subunit B antibody, "Transcription factor NF-Y, A subunit antibody", CAAT-box DNA binding protein subunit A antibody, HAP2 CCAAT-binding protein antibody, nuclear transcription factor Y subunit A antibody, nuclear transcription factor Y subunit alpha antibody, "nuclear transcription factor Y, alpha antibody"|
|Immunogen||Full length human NFYA Recombinant protein.|
|Species Reactivity||Human, Mouse, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Pig (97%), Rhesus Monkey (91%), Bovine (97%)|