|Applications||ICC/IF, IP, WB, ChIP assay|
Not tested in other applications.
| ChIP assay||Assay-dependent dilution
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||23 kDa (note)
|Positive Controls||HeLa nucleus , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||nuclear transcription factor Y, beta|
|Product Description||Rabbit Polyclonal antibody to NFYB (nuclear transcription factor Y, beta)|
|Background||The protein encoded by this gene is one subunit of a trimeric complex, forming a highly conserved transcription factor that binds with high specificity to CCAAT motifs in the promoter regions in a variety of genes. This gene product, subunit B, forms a tight dimer with the C subunit, a prerequisite for subunit A association. The resulting trimer binds to DNA with high specificity and affinity. Subunits B and C each contain a histone-like motif. Observation of the histone nature of these subunits is supported by two types of evidence; protein sequence alignments and experiments with mutants. [provided by RefSeq]|
|Synonyms||CBF-A antibody, CBF-B antibody, HAP3 antibody, NF-YB antibody, NFYB antibody, nuclear transcription factor Y subunit beta antibody, Transcription factor NF-Y, B subunit antibody, CAAT box DNA-binding protein subunit B antibody, CCAAT-binding transcription factor subunit A antibody, nuclear transcription factor Y subunit B antibody, nuclear transcription factor Y, beta antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human NFYB. The exact sequence is proprietary.|
|Species Reactivity||Human, Rat|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Mouse, Xenopus laevis, Pig, Rhesus Monkey, Bovine|
|Predict Reactivity Note||Mouse (98%), Xenopus laevis (92%), Pig (99%), Rhesus Monkey (100%), Bovine (98%)|