NOX2 / gp91phox antibody [NL7]
|Applications||ELISA, FACS, ICC/IF, IHC-Fr, WB|
| ELISA||Assay-dependent dilution
| Flow cytometry/FACS||Assay-dependent dilution
| Immunocytochemistry/ Immunofluorescence||Assay-dependent dilution
| IHC (Frozen sections)||Assay-dependent dilution
| Western blot||Assay-dependent dilution
Flow cytometry/FACS : Membrane permeabilisation is required for this application. Use 10μl of the suggested working dilution to label 106 cells in 100μl.Not tested in other applications.
Western blot : This antibody detects a broad band of approximately 90kDa.
*Optimal dilutions/concentrations should be determined by the researcher.
(Please refer to the vial label for the specific concentration)|
|Purification||Protein G purified|
|Purification Note||From tissue culture supernatant|
|Full Name||cytochrome b-245, beta polypeptide|
|Product Description||Mouse monoclonal antibody [NL7] to NOX2/gp91phox|
|Background||Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]|
|Synonyms||CYBB Antibody , CGD Antibody , GP91-PHOX Antibody , GP91PHOX Antibody , AMCBX2 Antibody , P91-PHOX Antibody , GP91-1 Antibody|
|Immunogen||Nox2 solubilised in octyl-beta-glucopyranoside.|