|Product Description||NP-40 (25%)|
|Background||Non ionic detergents like NP40 are used in immunoassays for diluting antibodies to lower background, and non-specific antigen-antibody (ag-ab) reaction. This detergent is also used in washing buffer from 0.1- 0.5% in all immunoassays (ELISA, Immunohistochemistry (IHC), Blot assays and others) to reduce background. Tergitol-type NP-40, which is nonyl phenoxylpolyethoxylethanol, lower surface tension of water.|
|Applications||ELISA, ICC/IF, IHC, WB|
|Application Note||For diluting antibodies use 1:1000 in buffers (1 ml into 999 ml).|
Washing buffer use 1:500 (1 ml into 499 ml).
The optimum dilution should be determined by the individual lab.
|Storage Buffer||This detergent is supplied as 25% in water with 0.05 % preservative.|
|Storage Instruction||Room temperature.|
|Notes||For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.|