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1 .GTX105741 NPAS2 antibody [C1C3]

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NPAS2 antibody [C1C3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-Fr, IHC-P, WB
  • Species reactivityHuman, Mouse, Rat
Cat No: GTX105741
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-NPAS2 antibody [C1C3] used in IHC (Frozen sections). GTX105741
  • Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
  • Anti-NPAS2 antibody [C1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX105741
  • Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
  • Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
  • Anti-NPAS2 antibody [C1C3] used in IHC (Frozen sections). GTX105741
  • Anti-NPAS2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105741
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  • Application Information: NPAS2 antibody [C1C3]

Catalog Number GTX105741
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Product Name NPAS2 antibody [C1C3]
See all NPAS2 products
ApplicationsICC/IF, IHC-Fr, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Frozen sections)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size92 kDa (note)
Positive ControlsA431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain
Form SuppliedLiquid
Concentration0.3 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: NPAS2 antibody [C1C3]

Full Nameneuronal PAS domain protein 2
Product DescriptionRabbit polyclonal antibody to NPAS2 (neuronal PAS domain protein 2)
BackgroundThe protein encoded by this gene is a member of the basic helix-loop-helix (bHLH)-PAS family of transcription factors. A similar mouse protein may play a regulatory role in the acquisition of specific types of memory. It also may function as a part of a molecular clock operative in the mammalian forebrain. [provided by RefSeq]
SynonymsFLJ23138 antibody, MGC71151 antibody, MOP4 antibody, PASD4 antibody, bHLHe9 antibody, NPAS2 antibody, class E basic helix-loop-helix protein 9 antibody, neuronal PAS domain-containing protein 2 antibody, PAS domain-containing protein 4 antibody, neuronal PAS2 antibody, basic-helix-loop-helix-PAS protein MOP4 antibody, member of PAS superfamily 4 antibody, member of PAS protein 4 antibody, neuronal PAS domain protein 2 antibody
Cellular LocalizationNucleus
HostRabbit
ClonalityPolyclonal
TargetNPAS2
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the C-terminus region of human NPAS2. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse, Rat
ConjugationUnconjugated

  • Storage Conditions: NPAS2 antibody [C1C3]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Type of cancer > Breast > Other
  • Cancer > Type of cancer > Prostate cancer
  • Neuroscience > Circadian rhythm
  • Stem Cell Development > Development > Ectoderm > Nervous system development
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NPAS2 antibody [C1C3] validated data
  • Anti-NPAS2 antibody [C1C3] used in IHC (Frozen sections). GTX105741
    • GTX105741 IHC-Fr Image
      NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis.
      Sample: Frozen sectioned E13.5 Rat brain.
      Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
      Blue: Fluoroshield with DAPI (GTX30920).
      Top
  • Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
    • GTX105741 WB Image
      Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] (GTX105741) diluted at 1:1000.
      Top
  • Anti-NPAS2 antibody [C1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX105741
    • GTX105741 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NPAS2(GTX105741) antibody at 1:100 dilution.
      Top
  • Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
    • GTX105741 WB Image
      Sample (30 ug of whole cell lysate)
      A: H1299
      B: Hela
      C: Hep G2 (GTX27900)
      7.5% SDS PAGE
      GTX105741 diluted at 1:1000
      Top
  • Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
    • GTX105741 WB Image
      Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] (GTX105741) diluted at 1:1000.
      Top
  • Anti-NPAS2 antibody [C1C3] used in IHC (Frozen sections). GTX105741
    • GTX105741 IHC-Fr Image
      NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis.
      Sample: Frozen sectioned adult mouse retina.
      Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
      Blue: Fluoroshield with DAPI (GTX30920).
      Top
  • Anti-NPAS2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105741
    • GTX105741 ICC/IF Image
      NPAS2 antibody [C1C3] detects NPAS2 protein at nucleus by immunofluorescent analysis.
      Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:500.
      Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
      Scale bar = 10 μm.
      Top
  • Anti-NPAS2 antibody [C1C3] used in IHC (Frozen sections). GTX105741
    • GTX105741 IHC-Fr Image
      NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis.
      Sample: Frozen sectioned E13.5 Rat brain.
      Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
      Blue: Fluoroshield with DAPI (GTX30920).
      Top
  • Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
    • GTX105741 WB Image
      Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] (GTX105741) diluted at 1:1000.
      Top
  • Anti-NPAS2 antibody [C1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX105741
    • GTX105741 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded H441 xenograft, using NPAS2(GTX105741) antibody at 1:100 dilution.
      Top
  • Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
    • GTX105741 WB Image
      Sample (30 ug of whole cell lysate)
      A: H1299
      B: Hela
      C: Hep G2 (GTX27900)
      7.5% SDS PAGE
      GTX105741 diluted at 1:1000
      Top
  • Anti-NPAS2 antibody [C1C3] used in Western Blot (WB). GTX105741
    • GTX105741 WB Image
      Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NPAS2 antibody [C1C3] (GTX105741) diluted at 1:1000.
      Top
  • Anti-NPAS2 antibody [C1C3] used in IHC (Frozen sections). GTX105741
    • GTX105741 IHC-Fr Image
      NPAS2 antibody [C1C3] detects NPAS2 protein expression by immunohistochemical analysis.
      Sample: Frozen sectioned adult mouse retina.
      Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:250.
      Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
      Blue: Fluoroshield with DAPI (GTX30920).
      Top
  • Anti-NPAS2 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX105741
    • GTX105741 ICC/IF Image
      NPAS2 antibody [C1C3] detects NPAS2 protein at nucleus by immunofluorescent analysis.
      Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
      Green: NPAS2 protein stained by NPAS2 antibody [C1C3] (GTX105741) diluted at 1:500.
      Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.
      Scale bar = 10 μm.
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensNPAS24862Q99743, A2I2P5603347NM_002518NP_00250991.82
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf

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