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1 .GTX115247 NPEPPS antibody [N3C2-2], Internal

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NPEPPS antibody [N3C2-2], Internal


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityHuman, Mouse
Cat No: GTX115247
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-NPEPPS antibody [N3C2-2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115247
  • Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247
  • Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247
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  • Application Information: NPEPPS antibody [N3C2-2], Internal

Catalog Number GTX115247
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Product Name NPEPPS antibody [N3C2-2], Internal
See all NPEPPS products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size103 kDa (note) IsoForm (3)
Positive ControlsA549 , HeLa , HepG2 , HCT116 , mouse brain
Form SuppliedLiquid
Concentration0.38 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: NPEPPS antibody [N3C2-2], Internal

Full Nameaminopeptidase puromycin sensitive
BackgroundThis gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq]
SynonymsAAP-S antibody, MP100 antibody, PSA antibody, NPEPPS antibody, puromycin-sensitive aminopeptidase antibody, cytosol alanyl aminopeptidase antibody, metalloproteinase MP100 antibody, aminopeptidase puromycin sensitive antibody
Cellular LocalizationCytoplasm (Potential) , Nucleus
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human NPEPPS. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Bovine
Predict Reactivity NoteRat (97%), Bovine (96%)
ConjugationUnconjugated

  • Storage Conditions: NPEPPS antibody [N3C2-2], Internal

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
NPEPPS antibody [N3C2-2], Internal validated data
  • Anti-NPEPPS antibody [N3C2-2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115247
    • GTX115247 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NPEPPS(GTX115247) antibody at 1:200 dilution.
      Top
  • Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247
    • GTX115247 WB Image
      Sample (50 µg of whole cell lysate)
      A: Mouse brain
      7.5% SDS PAGE
      GTX115247 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247
    • GTX115247 WB Image
      Sample (30 µg of whole cell lysate)
      A: HeLa
      7.5% SDS PAGE
      GTX115247 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-NPEPPS antibody [N3C2-2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115247
    • GTX115247 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NPEPPS(GTX115247) antibody at 1:200 dilution.
      Top
  • Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247
    • GTX115247 WB Image
      Sample (50 µg of whole cell lysate)
      A: Mouse brain
      7.5% SDS PAGE
      GTX115247 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-NPEPPS antibody [N3C2-2], Internal used in Western Blot (WB). GTX115247
    • GTX115247 WB Image
      Sample (30 µg of whole cell lysate)
      A: HeLa
      7.5% SDS PAGE
      GTX115247 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensNPEPPS9520P55786606793NP_001317186103
Homo sapiensNPEPPS9520P55786606793BC065294AAH6529498.52
Homo sapiensNPEPPS9520P55786606793NM_006310NP_006301103.29

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Mus musculusNpepps19155Q5PR74NM_008942NP_032968103.34
Rattus norvegicusNpepps50558Q8VID2NM_080395NP_536320103.36
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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