|Applications||ICC/IF, IHC-P, IP, WB|
Not tested in other applications.
|IHC (Formalin-fixed paraffin-embedded sections)||1:100-1:1000*
*Optimal dilutions/concentrations should be determined by the researcher.
|Predicted Target Size||54 kDa (note)
|Positive Controls||293T , 293T nuclear , A431 , A431 nuclear , HeLa , HeLa nuclear , HepG2 , HepG2 nuclear|
(Please refer to the vial label for the specific concentration)|
|Purification||Purified by antigen-affinity chromatography.|
|Full Name||nuclear respiratory factor 1|
|Product Description||Rabbit Polyclonal antibody to NRF1 (nuclear respiratory factor 1)|
|Background||This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq]|
|Synonyms||ALPHA-PAL antibody, NRF1 antibody, NRF-1 antibody, nuclear respiratory factor 1 antibody, alpha palindromic-binding protein antibody|
|Immunogen||Recombinant protein encompassing a sequence within the center region of human NRF1. The exact sequence is proprietary.|
|Species Reactivity||Human, Mouse|
|Predicted Cross Reactivity species Predicted cross-reactivity:|
Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
|Rat, Zebrafish, Xenopus laevis, Chicken, Bovine|
|Predict Reactivity Note||Rat (100%), Zebrafish (92%), Xenopus laevis (93%), Chicken (99%), Bovine (100%)|