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NRF2 antibody [N2C2], Internal

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in ChIP assay (ChIP assay). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322
Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

Cat. No. GTX103322

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, IP, ChIP assay

Reactivity

Human, Mouse, Rat, Zebrafish, Alligator, Plant, Bird
Package
100 μl ($399),
25 μl ($169)
PRODUCT

Summary

NRF2 antibody recognizes NRF2 protein, a basic leucine zipper region (bZip) transcription factor (predicted molecular weight of 68 kDa) that regulates expression of proteins with antioxidant and detoxification functions. NRF2 antibodies are commonly used to study oxidative stress, cell damage, metabolism, and immune response mechanisms, among others.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
FACS Assay dependent
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

68 kDa. ( Note )

Observed MW

110 kDa. The observed M.W. is based on the following publication. PMID: 22703241

Positive Control

3xFlag-human NFE2L2-transfected 293T , MDA-MB-231 nuclear extract , MDA-MB-231 nuclear extract (30uM tBHQ 4hr) , RAW264.7 (500 ng/ml LPS treatment for 6 hr)

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.65 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human NRF2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950993

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nuclear factor, erythroid 2 like 2 , HEBP1 , IMDDHH , NRF2

Cellular Localization

Cytoplasm , cytosol , Nucleus

Background

NFE2 (MIM 601490), NFE2L1 (MIM 163260), and NFE2L2 comprise a family of human genes encoding basic leucine zipper (bZIP) transcription factors. They share highly conserved regions that are distinct from other bZIP families, such as JUN (MIM 165160) and FOS (MIM 164810), although remaining regions have diverged considerably from each other (Chan et al., 1995 [PubMed 7868116]).[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Untreated (–) and treated (+) MDA-MB-231 nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.

Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322

GTX103322 ICC/IF Image

NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: NIH/3T3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 protein stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

NRF2 antibody detects NRF2 protein by western blot analysis. Non-transfected (-) and NRF2-transfected (+, including 3xFlag-tag) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody (GTX103322) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX103322

GTX103322 IHC-P Image

NRF2 antibody [N2C2], Internal detects NRF2 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-NRF2 antibody [N2C2], Internal used in ChIP assay (ChIP assay). GTX103322

GTX103322 ChIP assay Image

ChIP was performed with HepG2 chromatin extract and 5 μg of either normal rabbit IgG or anti-NRF2 antibody. The precipitated DNA was detected by PCR with primer set targeting to GCLC gene locus.

Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322

GTX103322 ICC/IF Image

NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Neuro2A cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.

Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322

GTX103322 ICC/IF Image

NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-NRF2 antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX103322

GTX103322 ICC/IF Image

NRF2 antibody [N2C2], Internal detects NRF2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: NRF2 stained by NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Untreated (–) and treated (+) RAW264.7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX103322

GTX103322 IP Image

Immunoprecipitation of NRF2 protein from HepG2 whole cell extracts using 5 μg of NRF2 antibody [N2C2], Internal (GTX103322).
Western blot analysis was performed using NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:500.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Untreated (–) and treated (+) Rat2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-NRF2 antibody [N2C2], Internal used in Western Blot (WB). GTX103322

GTX103322 WB Image

Untreated (–) and treated (+) Neuro2A whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NRF2 antibody [N2C2], Internal (GTX103322) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

REVIEW

NRF2 antibody [N2C2], Internal Cat. No. GTX103322

Rating ( Average 5 based on 8 users reviews)
Application
Western Blot(WB) ( Average 5 based on 7 users reviews)
IHC (Paraffin sections)(IHC-P) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Nov-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse hepatocyte
Amount used : 50μg
Blocking : 5% nonfat milk, 20°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Date : Anonymous submitted on 19-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : LNCaP cells
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, RT°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description :
Lane1: Control (24 h after irradiation with 0 Gy)
Lane2: 24 h after irradiation with 2 Gy
Lane3: 24 h after irradiation with 4 Gy
Lane4: 24 h after irradiation with 8 Gy
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27023634
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016.PMID: 27023634
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Figure Note : The data was peer reviewed and published in the journal Redox Biol in 2017.PMID: 28448946
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Int J Mol Sci in 2017. PMID: 28926934
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2013. PMID: 24204994
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Cells in 2019. PMID: 31366086
Application Reference
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169