Dengue virus NS2B protein antibody

Host / ClonalityRabbit Polyclonal
ApplicationsICC/IF, WB
Species reactivityDengue virus
★★★★★ ( 2 ) ( 12 )

Cat No:

GTX124246

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

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Datasheet
Star Review (2)
Reference (12)
Protocol & MSDS

Application Information: Dengue virus NS2B protein antibody

Catalog Number

GTX124246

Product Name

Dengue virus NS2B protein antibody

See all Dengue virus NS2B products

Applications

ICC/IF, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:2000*	Y
Western Blot (WB)	1:1000-1:10000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

15 kDa (note)

Positive Controls

Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , *Dengue virus 1 infected C6/36 mosquito , *Dengue virus 2 infected Huh-7

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Dengue virus NS2B protein antibody

Full Name	non-structural protein 2B (Dengue virus 2)
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Full length Dengue virus 2 NS2B Recombinant protein
Antigen Species	Dengue virus 2
Species Reactivity	Dengue virus
Cross Reactivity Note	Though raised against DEN2 protein, this antibody is predicted to cross-react with the other serotypes based on comparative sequence analysis. Experimental evidence from an independent lab showed that this antibody does detect the target protein by western blot in DEN1-infected C6/36 mosquito cell lysates (please see "Star Review").
Conjugation	Unconjugated

Storage Conditions: Dengue virus NS2B protein antibody

Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20° C or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Infectious Disease > Virus > Dengue Virus (DEV) More Hide

Dengue virus NS2B protein antibody validated data

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124246 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124246 WB Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein by western blot analysis.
A. 66 µg BHK-21 whole cell extract
B. 20 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 10 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
15% SDS-PAGE
NS2B (Dengue virus) antibody (GTX124246) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124246 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124246 WB Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein by western blot analysis.
A. 66 µg BHK-21 whole cell extract
B. 20 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 10 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
15% SDS-PAGE
NS2B (Dengue virus) antibody (GTX124246) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

StarReview
Dengue virus NS2B protein antibody
Rating : ★★★★★ ( Average 5.0 based on 2 users reviews)
Western Blot (WB)(2) :★★★★★

Anonymous StarReview submitted on 21 Nov 2013
Score : ★★★★★
Application Tested : WB
Sample Species : Dengue virus
Sample : Dengue virus 1 infected C6/36 mosquito cells
Amount used(ug/cells) : 30ug
Blocking conditions
Blocking Buffer : 5% BSA in 1x TBS
Incubation Time & Temperature : RT (℃)/1hr
Primary antibody
Dilution : 1:10000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight
Data Description
Lane A:Mock Lane B:Dengue virus 1 infected C6/36 mosquito cells

Anonymous StarReview submitted on 20 Jan 2014
Score : ★★★★★
Application Tested : WB
Sample Species : Dengue virus
Sample : Dengue virus 2 infected Huh-7 cells
Amount used(ug/cells) : 10ug
Blocking conditions
Blocking Buffer : 5% skin milk
Incubation Time & Temperature : RT (℃)/3hr
Primary antibody
Dilution : 1:3000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight
Data Description
Lane1:Huh-7 cells Lane2:Dengue virus 2 infected Huh-7 cells

Reference for Dengue virus NS2B protein antibody

Application References	Application	Reactivity
Lai JH et al. Infection with the dengue RNA virus activates TLR9 signaling in human dendritic cells. EMBO Rep (Epub ahead of print) (2018).	ICC/IF
Saisawang C et al. Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities. PLoS One 13 (2):e0193133 (2018).	WB
Reid DW et al. Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis. J Virol (Epub ahead of print) (2018).	WB
Lee JC et al. Discovery of novel diarylpyrazolylquinoline derivatives as potent anti-dengue virus agents. European Journal of Medicinal Chemistry 141:282-292 (2017).	WB
Yu JS et al. Schisandrin A inhibits dengue viral replication via upregulating antiviral interferon responses through STAT signaling pathway. Sci Rep 7:45171 (2017).	WB
Lin CK et al. Cyclooxygenase-2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents. Sci Rep 7:44701 (2017).	WB
Yu JS et al. Celastrol inhibits dengue virus replication via up-regulating type I interferon and downstream interferon-stimulated responses. Antiviral Res 137:49-57 (2016).	WB
Tseng CK et al. Human heme oxygenase 1 is a potential host cell factor against dengue virus replication. Sci Rep 6:32176 (2016).	WB
Lin CK et al. Butyrolactones and Diketopiperazines from Marine Microbes: Inhibition Effects on Dengue Virus Type 2 Replication. Planta Med (2016).	WB
Marceau C.D. et al. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature 535 (7610):159-63 (2016).	WB	Dengue Virus
Lee JC et al. Characterization of the activity of 2'-C-methylcytidine against dengue virus replication. Antiviral Res 116:1-9 (2015).	WB	Dengue virus
Tseng CH et al. Synthesis, antiproliferative and anti-dengue virus evaluations of 2-aroyl-3-arylquinoline derivatives. Eur J Med Chem 79C:66-76 (2014).	WB	Dengue virus