Dengue virus NS2B protein antibody

Host / ClonalityRabbit Polyclonal
ApplicationsICC/IF, WB
Species reactivityDengue virus
★★★★★ ( 2 ) ( 11 )

Cat No:

GTX124246

Package	Price	Qty
25 μl	USD 119.00
100 μl	USD 319.00

Add to cart

LinkButton

Datasheet
Star Review (2)
Reference (11)
Protocol & MSDS

Application Information: Dengue virus NS2B protein antibody

Catalog Number

GTX124246

Product Name

Dengue virus NS2B protein antibody

See all Dengue virus NS2B products

Applications

ICC/IF, WB

Application Note

	Suggested dilution	Reference
Immunocytochemistry/ Immunofluorescence (ICC/IF)	1:100-1:2000*
Western Blot (WB)	1:1000-1:10000*	Y

Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.

Predicted Target Size

15 kDa (note)

Positive Controls

Dengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , *Dengue virus 1 infected C6/36 mosquito , *Dengue virus 2 infected Huh-7

Form Supplied

Liquid

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration)

Purification

Purified by antigen-affinity chromatography.

Specifications: Dengue virus NS2B protein antibody

Full Name	non-structural protein 2B (Dengue virus 2)
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Full length Dengue virus 2 NS2B Recombinant protein
Antigen Species	Dengue virus 2
Species Reactivity	Dengue virus
Cross Reactivity Note	Though raised against DEN2 protein, this antibody is predicted to cross-react with the other serotypes based on comparative sequence analysis. Experimental evidence from an independent lab showed that this antibody does detect the target protein by western blot in DEN1-infected C6/36 mosquito cell lysates (please see "Star Review").
Conjugation	Unconjugated

Storage Conditions: Dengue virus NS2B protein antibody

Storage Buffer	1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage Instruction	Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Notes	For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
Infectious Disease > Virus > Dengue Virus (DEV) More Hide

Dengue virus NS2B protein antibody validated data

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124246 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124246 WB Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein by western blot analysis.
A. 66 µg BHK-21 whole cell extract
B. 20 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 10 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
15% SDS-PAGE
NS2B (Dengue virus) antibody (GTX124246) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124246 WB Image

Sample (20 µg of whole cell lysate)
A: BHK-21
B: Dengue virus 2 infect BHK-21
C: Japanese encephalitis virus infect BHK-21
15% SDS PAGE
GTX124246 diluted at 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Top

GTX124246 ICC/IF Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
Blue: Hoechst 33342 staining.

Top

GTX124246 WB Image

NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein by western blot analysis.
A. 66 µg BHK-21 whole cell extract
B. 20 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
C. 10 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
15% SDS-PAGE
NS2B (Dengue virus) antibody (GTX124246) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

The results may vary from different DENV strains, viral infection status, and culture conditions.
Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.

Top

StarReview
Dengue virus NS2B protein antibody
Rating : ★★★★★ ( Average 5.0 based on 2 users reviews)
Western Blot (WB)(2) :★★★★★

Anonymous StarReview submitted on 21 Nov 2013
Score : ★★★★★
Application Tested : WB
Sample Species : Dengue virus
Sample : Dengue virus 1 infected C6/36 mosquito cells
Amount used(ug/cells) : 30ug
Blocking conditions
Blocking Buffer : 5% BSA in 1x TBS
Incubation Time & Temperature : RT (℃)/1hr
Primary antibody
Dilution : 1:10000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight
Data Description
Lane A:Mock Lane B:Dengue virus 1 infected C6/36 mosquito cells

Anonymous StarReview submitted on 20 Jan 2014
Score : ★★★★★
Application Tested : WB
Sample Species : Dengue virus
Sample : Dengue virus 2 infected Huh-7 cells
Amount used(ug/cells) : 10ug
Blocking conditions
Blocking Buffer : 5% skin milk
Incubation Time & Temperature : RT (℃)/3hr
Primary antibody
Dilution : 1:3000( conc. of dilution )
Incubation Time & Temperature : 4 (℃)/overnight
Data Description
Lane1:Huh-7 cells Lane2:Dengue virus 2 infected Huh-7 cells

Reference for Dengue virus NS2B protein antibody

Application References	Application	Reactivity
Saisawang C et al. Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities. PLoS One 13 (2):e0193133 (2018).	WB
Reid DW et al. Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis. J Virol (Epub ahead of print) (2018).	WB
Lee JC et al. Discovery of novel diarylpyrazolylquinoline derivatives as potent anti-dengue virus agents. European Journal of Medicinal Chemistry 141:282-292 (2017).	WB
Yu JS et al. Schisandrin A inhibits dengue viral replication via upregulating antiviral interferon responses through STAT signaling pathway. Sci Rep 7:45171 (2017).	WB
Lin CK et al. Cyclooxygenase-2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents. Sci Rep 7:44701 (2017).	WB
Yu JS et al. Celastrol inhibits dengue virus replication via up-regulating type I interferon and downstream interferon-stimulated responses. Antiviral Res 137:49-57 (2016).	WB
Tseng CK et al. Human heme oxygenase 1 is a potential host cell factor against dengue virus replication. Sci Rep 6:32176 (2016).	WB
Lin CK et al. Butyrolactones and Diketopiperazines from Marine Microbes: Inhibition Effects on Dengue Virus Type 2 Replication. Planta Med (2016).	WB
Marceau C.D. et al. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature 535 (7610):159-63 (2016).	WB	Dengue Virus
Lee JC et al. Characterization of the activity of 2'-C-methylcytidine against dengue virus replication. Antiviral Res 116:1-9 (2015).	WB	Dengue virus
Tseng CH et al. Synthesis, antiproliferative and anti-dengue virus evaluations of 2-aroyl-3-arylquinoline derivatives. Eur J Med Chem 79C:66-76 (2014).	WB	Dengue virus