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1 .GTX124246 Dengue virus NS2B protein antibody

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Dengue virus NS2B protein antibody


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, WB
  • Species reactivityDengue virus
  • ★★★★★   (  2  ) Publication  ( 11 )
Cat No: GTX124246
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
  • Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
  • Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
  • Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
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  • Datasheet
  • Star Review (2)
    Reference (11)
  • Protocol & MSDS
  • Application Information: Dengue virus NS2B protein antibody

Catalog Number GTX124246
★★★★★
( 2 )
Publication ( 11 )
Product Name Dengue virus NS2B protein antibody
See all Dengue virus NS2B products
ApplicationsICC/IF, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:2000*  
Western Blot (WB)1:1000-1:10000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size15 kDa (note)
Positive ControlsDengue virus type 2 infected BHK-21 , Dengue virus type 3 infected BHK-21 , *Dengue virus 1 infected C6/36 mosquito , *Dengue virus 2 infected Huh-7
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: Dengue virus NS2B protein antibody

Full Namenon-structural protein 2B (Dengue virus 2)
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenFull length Dengue virus 2 NS2B Recombinant protein
Antigen SpeciesDengue virus 2
Species ReactivityDengue virus
Cross Reactivity NoteThough raised against DEN2 protein, this antibody is predicted to cross-react with the other serotypes based on comparative sequence analysis. Experimental evidence from an independent lab showed that this antibody does detect the target protein by western blot in DEN1-infected C6/36 mosquito cell lysates (please see "Star Review").
ConjugationUnconjugated

  • Storage Conditions: Dengue virus NS2B protein antibody

Storage Buffer1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Infectious Disease > Virus > Dengue Virus (DEV)
More Hide
Dengue virus NS2B protein antibody validated data
  • Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
    • GTX124246 ICC/IF Image
      NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
      Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
    • GTX124246 WB Image
      Sample (20 µg of whole cell lysate)
      A: BHK-21
      B: Dengue virus 2 infect BHK-21
      C: Japanese encephalitis virus infect BHK-21
      15% SDS PAGE
      GTX124246 diluted at 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
    • GTX124246 ICC/IF Image
      NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
      Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
    • GTX124246 WB Image
      NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein by western blot analysis.
      A. 66 µg BHK-21 whole cell extract
      B. 20 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
      C. 10 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
      15% SDS-PAGE
      NS2B (Dengue virus) antibody (GTX124246) dilution: 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      The results may vary from different DENV strains, viral infection status, and culture conditions.
      Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.
      Top
  • Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
    • GTX124246 ICC/IF Image
      NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 3 (right) were fixed in MeOH.
      Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
    • GTX124246 WB Image
      Sample (20 µg of whole cell lysate)
      A: BHK-21
      B: Dengue virus 2 infect BHK-21
      C: Japanese encephalitis virus infect BHK-21
      15% SDS PAGE
      GTX124246 diluted at 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-Dengue virus NS2B protein antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124246
    • GTX124246 ICC/IF Image
      NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein at cytoplasm by immunofluorescent analysis.
      Samples: BHK-21 cells mock (left) and infected with Dengue virus 2 (right) were fixed in MeOH.
      Green: NS2B (Dengue virus) protein stained by NS2B (Dengue virus) antibody (GTX124246) diluted at 1:2000.
      Blue: Hoechst 33342 staining.
      Top
  • Anti-Dengue virus NS2B protein antibody used in Western Blot (WB). GTX124246
    • GTX124246 WB Image
      NS2B (Dengue virus) antibody detects NS2B (Dengue virus) protein by western blot analysis.
      A. 66 µg BHK-21 whole cell extract
      B. 20 µg whole cell extract of Dengue virus type 2 infected BHK-21 cells
      C. 10 µg whole cell extract of Dengue virus type 3 infected BHK-21 cells
      15% SDS-PAGE
      NS2B (Dengue virus) antibody (GTX124246) dilution: 1:5000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      The results may vary from different DENV strains, viral infection status, and culture conditions.
      Reference: Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):6795-9, J Infect Dis. 2011 Jun 15;203(12):1775-83.
      Top
  • StarReview
  • Dengue virus NS2B protein antibody

  • Rating : ★★★★★ ( Average 5.0 based on 2 users reviews)
  • Western Blot (WB)(2) :★★★★★
  • Anonymous StarReview submitted on 21 Nov 2013
  • Score : ★★★★★
  • Application Tested : WB
  • Sample Species : Dengue virus
  • Sample : Dengue virus 1 infected C6/36 mosquito cells
  • Amount used(ug/cells) : 30ug
  • Blocking conditions
  • Blocking Buffer : 5% BSA in 1x TBS
  • Incubation Time & Temperature : RT (℃)/1hr
  • Primary antibody
  • Dilution : 1:10000( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/overnight
  • Data Description
  • Lane A:Mock Lane B:Dengue virus 1 infected C6/36 mosquito cells
  • Anonymous StarReview submitted on 20 Jan 2014
  • Score : ★★★★★
  • Application Tested : WB
  • Sample Species : Dengue virus
  • Sample : Dengue virus 2 infected Huh-7 cells
  • Amount used(ug/cells) : 10ug
  • Blocking conditions
  • Blocking Buffer : 5% skin milk
  • Incubation Time & Temperature : RT (℃)/3hr
  • Primary antibody
  • Dilution : 1:3000( conc. of dilution )
  • Incubation Time & Temperature : 4 (℃)/overnight
  • Data Description
  • Lane1:Huh-7 cells Lane2:Dengue virus 2 infected Huh-7 cells
Back to Top
  • Reference for Dengue virus NS2B protein antibody
Application References Application Reactivity
Saisawang C et al. Glutathionylation of dengue and Zika NS5 proteins affects guanylyltransferase and RNA dependent RNA polymerase activities. PLoS One 13 (2):e0193133 (2018). WB
Reid DW et al. Dengue virus selectively annexes endoplasmic reticulum-associated translation machinery as a strategy for co-opting host cell protein synthesis. J Virol (Epub ahead of print) (2018). WB
Lee JC et al. Discovery of novel diarylpyrazolylquinoline derivatives as potent anti-dengue virus agents. European Journal of Medicinal Chemistry 141:282-292 (2017). WB
Yu JS et al. Schisandrin A inhibits dengue viral replication via upregulating antiviral interferon responses through STAT signaling pathway. Sci Rep 7:45171 (2017). WB
Lin CK et al. Cyclooxygenase-2 facilitates dengue virus replication and serves as a potential target for developing antiviral agents. Sci Rep 7:44701 (2017). WB
Yu JS et al. Celastrol inhibits dengue virus replication via up-regulating type I interferon and downstream interferon-stimulated responses. Antiviral Res 137:49-57 (2016). WB
Tseng CK et al. Human heme oxygenase 1 is a potential host cell factor against dengue virus replication. Sci Rep 6:32176 (2016). WB
Lin CK et al. Butyrolactones and Diketopiperazines from Marine Microbes: Inhibition Effects on Dengue Virus Type 2 Replication. Planta Med (2016). WB
Marceau C.D. et al. Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens. Nature 535 (7610):159-63 (2016). WB Dengue Virus
Lee JC et al. Characterization of the activity of 2'-C-methylcytidine against dengue virus replication. Antiviral Res 116:1-9 (2015). WB Dengue virus
Tseng CH et al. Synthesis, antiproliferative and anti-dengue virus evaluations of 2-aroyl-3-arylquinoline derivatives. Eur J Med Chem 79C:66-76 (2014). WB Dengue virus
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-PBS.pdf
    MSDS-Thimerosal.pdf
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