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  • NSFL1C antibody [N1C2]

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1 .GTX105035 NSFL1C antibody [N1C2]

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NSFL1C antibody [N1C2]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsIHC-P, IP, WB
  • Species reactivityHuman, Mouse
Cat No: GTX105035
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
Add to cart
 
  • Anti-NSFL1C antibody [N1C2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX105035
  • Anti-NSFL1C antibody [N1C2] used in Immunoprecipitation. GTX105035
  • Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035
  • Anti-NSFL1C antibody [N1C2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX105035
  • Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035
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  • Application Information: NSFL1C antibody [N1C2]

Catalog Number GTX105035
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Product Name NSFL1C antibody [N1C2]
See all NSFL1C products
ApplicationsIHC-P, IP, WB
Application Note
Suggested dilutionReference
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Immunoprecipitation1:100-1:500*  
Western Blot (WB)1:500-1:3000*  
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size41 kDa (note) IsoForm (2)
Positive Controls293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain
Form SuppliedLiquid
Concentration1 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: NSFL1C antibody [N1C2]

Full NameNSFL1 (p97) cofactor (p47)
BackgroundN-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]
SynonymsMGC3347 antibody, UBX1 antibody, UBXD10 antibody, UBXN2C antibody, dJ776F14.1 antibody, p47 antibody, NSFL1C antibody, NSFL1 cofactor p47 antibody, SHP1 homolog antibody, UBX domain-containing protein 2C antibody, p97 cofactor p47 antibody, NSFL1 (p97) cofactor (p47) antibody
Cellular LocalizationNucleus (By similarity) , Golgi apparatus , Golgi stack (By similarity)
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human NSFL1C. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman, Mouse
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Rat, Xenopus laevis, Pig, Chicken, Rhesus Monkey, Bovine
Predict Reactivity NoteRat (96%), Xenopus laevis (83%), Pig (98%), Chicken (87%), Rhesus Monkey (100%), Bovine (96%)
ConjugationUnconjugated

  • Storage Conditions: NSFL1C antibody [N1C2]

Storage Buffer0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
NSFL1C antibody [N1C2] validated data
  • Anti-NSFL1C antibody [N1C2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX105035
    • GTX105035 IHC-P Image
      NSFL1C antibody [N1C2] detects NSFL1C protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse brain.
      NSFL1C antibody [N1C2] (GTX105035) diluted at 1:500.
      Top
  • Anti-NSFL1C antibody [N1C2] used in Immunoprecipitation. GTX105035
    • GTX105035 IP Image
      Immunoprecipitation of NSFL1C protein from A431 whole cell extracts using 5 μg of NSFL1C antibody [N1C2] (GTX105035).
      Western blot analysis was performed using NSFL1C antibody [N1C2] (GTX105035).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035
    • GTX105035 WB Image
      Sample (50 µg of whole cell lysate)
      A: Mouse brain
      10% SDS PAGE
      GTX105035 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-NSFL1C antibody [N1C2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX105035
    • GTX105035 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded differential N/T pair Lung SCC cancer xenograft, using NSFL1C(GTX105035) antibody at 1:100 dilution.
      Top
  • Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035
    • GTX105035 WB Image
      Sample(30 µg whole cell lysate)
      A:293T
      B:A431(GTX27909)
      C:H1299
      10% SDS PAGE
      GTX105035 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-NSFL1C antibody [N1C2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX105035
    • GTX105035 IHC-P Image
      NSFL1C antibody [N1C2] detects NSFL1C protein at cytoplasm and nucleus on mouse brain by immunohistochemical analysis.
      Sample: Paraffin-embedded mouse brain.
      NSFL1C antibody [N1C2] (GTX105035) diluted at 1:500.
      Top
  • Anti-NSFL1C antibody [N1C2] used in Immunoprecipitation. GTX105035
    • GTX105035 IP Image
      Immunoprecipitation of NSFL1C protein from A431 whole cell extracts using 5 μg of NSFL1C antibody [N1C2] (GTX105035).
      Western blot analysis was performed using NSFL1C antibody [N1C2] (GTX105035).
      EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
      Top
  • Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035
    • GTX105035 WB Image
      Sample (50 µg of whole cell lysate)
      A: Mouse brain
      10% SDS PAGE
      GTX105035 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
  • Anti-NSFL1C antibody [N1C2] used in IHC (Formalin-fixed paraffin-embedded sections). GTX105035
    • GTX105035 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded differential N/T pair Lung SCC cancer xenograft, using NSFL1C(GTX105035) antibody at 1:100 dilution.
      Top
  • Anti-NSFL1C antibody [N1C2] used in Western Blot (WB). GTX105035
    • GTX105035 WB Image
      Sample(30 µg whole cell lysate)
      A:293T
      B:A431(GTX27909)
      C:H1299
      10% SDS PAGE
      GTX105035 diluted at 1:1000
      The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
      Top
Back to Top
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensNSFL1C55968Q9UNZ2, Q5JXA4, B2RD74, A2A2L1606610NM_016143NP_05722740.58
Homo sapiensNSFL1C55968Q9UNZ2, Q5JXA4, B2RD74, A2A2L1606610NM_018839NP_06132737.33

Cross-react Bioinfo
SpeciesGene nameGene IDSwissProtIsoform (mRNA)Protein Accession#MW
Rattus norvegicusNsfl1c83809O35987NM_031981NP_11418740.69
Mus musculusNsfl1c386649Q3UVN5NP_00127800340.72
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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