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1 .GTX116398 NXF3 antibody [C1C3]

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NXF3 antibody [C1C3]


  • Host / ClonalityRabbit Polyclonal
  • ApplicationsICC/IF, IHC-P, WB
  • Species reactivityHuman
  • Publication  ( 1 )
Cat No: GTX116398
Package Price Qty
25 μlUSD 119.00
100 μlUSD 319.00
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  • Anti-NXF3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116398
  • Anti-NXF3 antibody [C1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX116398
  • Anti-NXF3 antibody [C1C3] used in Western Blot (WB). GTX116398
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  • Bio-info
  • Protocol & MSDS
  • Application Information: NXF3 antibody [C1C3]

Catalog Number GTX116398
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( 0 )
Publication ( 1 )
Product Name NXF3 antibody [C1C3]
See all NXF3 products
ApplicationsICC/IF, IHC-P, WB
Application Note
Suggested dilutionReference
Immunocytochemistry/ Immunofluorescence (ICC/IF)1:100-1:1000*  
IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000*  
Western Blot (WB)1:5000-1:20000* Y
Not tested in other applications.
*Optimal dilutions/concentrations should be determined by the researcher.
Predicted Target Size60 kDa (note) IsoForm (2)
Positive ControlsNT2D1 , IMR32 , U87-MG , MCF-7
Form SuppliedLiquid
Concentration0.95 mg/ml (Please refer to the vial label for the specific concentration)
PurificationPurified by antigen-affinity chromatography.

  • Specifications: NXF3 antibody [C1C3]

Full Namenuclear RNA export factor 3
BackgroundThis gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene has shortened LRR and ubiquitin-associated domains and its RDB is unable to bind RNA. It is located in the nucleoplasm but is not associated with either the nuclear envelope or the nucleolus. [provided by RefSeq]
SynonymsNXF3 antibody, TAPL-3 antibody, TAP-like protein 3 antibody, nuclear RNA export factor 3 antibody
Cellular LocalizationNucleus , Cytoplasm
HostRabbit
ClonalityPolyclonal
IsotypeIgG
ImmunogenRecombinant protein encompassing a sequence within the center region of human NXF3. The exact sequence is proprietary.
Antigen SpeciesHuman
Species ReactivityHuman
Predicted Cross Reactivity species Predicted cross-reactivity:
    Predicted cross-reactivity is based on sequence homology, with greater than 80% immunogen sequence identity considered positive.
    Please note that we are only able to guarantee products to work in applications and species in which they have been tested.
Chimpanzee
Predict Reactivity NoteChimpanzee (98%)
ConjugationUnconjugated

  • Storage Conditions: NXF3 antibody [C1C3]

Storage Buffer0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Storage InstructionKeep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
NXF3 antibody [C1C3] validated data
  • Anti-NXF3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116398
    • GTX116398 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using NXF3(GTX116398) antibody at 1:200 dilution.
      Top
  • Anti-NXF3 antibody [C1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX116398
    • GTX116398 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NXF3(GTX116398) antibody at 1:100 dilution.
      Top
  • Anti-NXF3 antibody [C1C3] used in Western Blot (WB). GTX116398
    • GTX116398 WB Image
      Sample (30 ug of whole cell lysate)
      A: NT2D1
      B: IMR32
      C: U87-MG
      D: MCF-7
      7.5% SDS PAGE
      GTX116398 diluted at 1:10000
      Top
  • Anti-NXF3 antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116398
    • GTX116398 ICC/IF Image
      Immunofluorescence analysis of paraformaldehyde-fixed MCF-7, using NXF3(GTX116398) antibody at 1:200 dilution.
      Top
  • Anti-NXF3 antibody [C1C3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX116398
    • GTX116398 IHC-P Image
      Immunohistochemical analysis of paraffin-embedded A549 xenograft, using NXF3(GTX116398) antibody at 1:100 dilution.
      Top
  • Anti-NXF3 antibody [C1C3] used in Western Blot (WB). GTX116398
    • GTX116398 WB Image
      Sample (30 ug of whole cell lysate)
      A: NT2D1
      B: IMR32
      C: U87-MG
      D: MCF-7
      7.5% SDS PAGE
      GTX116398 diluted at 1:10000
      Top
Back to Top
  • Reference for NXF3 antibody [C1C3]
Application References Application Reactivity
Sundararaman B et al. Resources for the Comprehensive Discovery of Functional RNA Elements. Mol Cell 61 (6):903-13 (2016). WB Human
Target Bioinfo/ Predicted Isoform Cross Reactivity
SpeciesGene nameGene IDSwissProtOMIMIsoform (mRNA)Protein Accession#MW
Homo sapiensNXF356000Q9H4D5, Q5H9I1300316BC031616AAH3161660.11
Homo sapiensNXF356000Q9H4D5, Q5H9I1300316NM_022052NP_07133560.11
    *MSDS
    MSDS-Glycerol.pdf
    MSDS-Thimerosal.pdf
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All products are for research use only—Not for use in diagnostic procedures.