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1 .GTX47979-PRO Nanog-TAT Recombinant Protein

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Nanog-TAT Recombinant Protein


  • ApplicationsELISA, WB
Cat No: GTX47979-PRO
Package Price Qty
20 μgUSD 319.00
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  • Application Information: Nanog-TAT Recombinant Protein

Catalog Number GTX47979-PRO
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Product Name Nanog-TAT Recombinant Protein
See all Nanog products
Full NameNanog homeobox
BackgroundNanog is a regulatory protein that is associated with undifferentiated pluripotent cells. The expression of Nanog, which is suppressed in all adult tissues, is restricted to embryonic stem cells and to certain pluripotent cancer cells. Decreased expression of Nanog is strongly correlated with cell differentiation. Nanog, most likely, acts as an intracellular regulator, which helps maintain pluripotency and self renewal via a STAT3 independent pathway. The introduction of Nanog, along with Sox2, Oct4, Lin28, into primary human fibroblasts was sufficient to confer a pluripotent state upon the fibroblast genome. The reprogrammed cells thus obtained resemble ESC in morphology and gene expression. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing transcription factors into primary as well as transformed cells. Recombinant human Nanog-TAT is a 36.2 kDa protein, which is synthesized as a 304 amino acid polypeptide plus a 13- residue C-terminal TAT peptide.
SpeciesHuman
Region/SequenceSVDPACPQSL PCFEASDCKE SSPMPVICGP EENYPSLQMS SAEMPHTETV SPLPSSMDLL IQDSPDSSTS PKGKQPTSAE NSVAKKEDKV PVKKQKTRTV FSSTQLCVLN DRFQRQKYLS LQQMQELSNI LNLSYKQVKT WFQNQRMKSK RWQKNNWPKN SNGVTQKASA PTYPSLYSSY HQGCLVNPTG NLPMWSNQTW NNSTWSNQTQ NIQSWSNHSW NTQTWCTQSW NNQAWNSPFY NCGEESLQSC MQFQPNSPAS DLEAALEAAG EGLNVIQQTT RYFSTPQTMD LFLNYSMNMQ PEDVGGYGRK KRRQRRR
Expression SystemE. coli
ApplicationsELISA, WB
Application NoteEndotoxin level is less than 0.1 ng per ug (1EU/ug).
ConjugationUnconjugated
Form SuppliedLyophilized powder
PurityGreater than 95% by SDS-PAGE gel and HPLC analyses. Endotoxin level is less than 0.1 ng per µg (1EU/µg).
ConcentrationBatch dependent (Please refer to the vial label for the specific concentration)
Storage InstructionThe lyophilized recombinant protein is stable for at least 2 years from date of receipt at -20˚C. After reconstitution, it is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. Avoid multiple freeze-thaw cycles. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.
NotesFor In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
ResearchArea
  • Cancer > Tumor biomarkers
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Nanog-TAT Recombinant Protein validated data
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All products are for research use only—Not for use in diagnostic procedures.